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Bhattacharjee, AnukanaCharacterization of the DNA Binding Properties of CST (CTC1-STN1-TEN1) And Their Importance for CST Function in Telomeric as well as Genome-wide Replication
PhD, University of Cincinnati, 2017, Medicine: Molecular Genetics, Biochemistry, & Microbiology
Telomeres are the protective DNA-protein complexes at the ends of chromosomes. Mammalian telomeres are composed of double-stranded DNA with a repetitive sequence (TTAGGG) followed by a short single-stranded overhang. The DNA is bound by a series of proteins that prevent chromosome fusions and protect the DNA terminus from being sensed as damage. These telomere proteins also aid in telomere replication. Shelterin, the primary telomere protein complex, binds to both the double- and single-strand regions of the telomere. Shelterin is important for protecting telomeres from being sensed as damage. It also brings in telomerase for telomere extension. The other major telomere protein complex is CST (CTC1-STN1-TEN1). CST functions in telomere replication first by aiding passage of the replication machinery through the telomere duplex and then enabling fill-in synthesis of the telomeric C-strand following telomerase action. CST also has genome-wide roles in the resolution of replication stress. CST resembles Replication Protein A (RPA) in that it binds ssDNA and STN1 and TEN1 are structurally similar to RPA2 and RPA3. Here we address CST mechanism by using STN1 OB-fold mutant (STN1-OBM) to examine the relationship between DNA binding and CST function. In vivo, STN1-OBM affects resolution of endogenous replication stress and telomere duplex replication but telomeric C-strand fill-in and new origin firing after exogenous replication stress are unaffected. In vitro binding studies show that STN1 directly engages both short and long ssDNA, however STN1-OBM preferentially destabilizes binding to short substrates. CST is expected to engage DNA substrates of varied length and structure as it acts to resolve different replication problems. Since STN1-OBM will alter CST binding to only some of these substrates, the mutant should affect resolution of only a subset of replication problems, as was observed in the STN1-OBM cells. The in vitro studies also provide insight into CST binding mechanism. Like RPA, CST likely contacts DNA via multiple OB folds. However, the importance of STN1 for binding short substrates indicates differences in the architecture of CST and RPA DNA-protein complexes. Although the architecture of DNA binding differ for RPA and CST, their overall structural similarity motivated us to use RPA as a model to investigate DNA binding properties underlying CST function. RPA binds to ssDNA with high affinity, yet individual OB-folds can micro-dissociate from the DNA promoting sliding of RPA on the DNA, melting of dsDNA or secondary structures, recruitment of interaction partners. By using single molecule fluorescence assays, we show that CST cannot melt dsDNA but it can resolve secondary structures such as G4. The efficiency of G4 unfolding by CST, and its known abundance in G-rich regions genome-wide could explain its role in resolution of replication stress. Our work has also shown that CST can recognize ss-dsDNA junctions which start to explain the incremental nature of the telomeric C-strand fill-in reaction after G-strand extension by telomerase. Overall, our work provides insight into the mechanism by which CST might resolve replication issues at the telomere and genome wide.

Committee:

Carolyn Price, Ph.D. (Committee Chair); Iain Cartwright, Ph.D. (Committee Member); Rhett Kovall, Ph.D. (Committee Member); Anil Menon, Ph.D. (Committee Member); Satoshi Namekawa, Ph.D. (Committee Member)

Subjects:

Biogeochemistry

Keywords:

Telomere;Replication;CTC1 STN1 TEN1;DNA binding;OB fold

Terway, SaakshiPreserving the Intangible Heritage: Neemrana Kala Kendra – A Crafts and Tourism Complex
MARCH, University of Cincinnati, 2017, Design, Architecture, Art and Planning: Architecture
With rapid modernization and globalization, as the world is coming closer together, it gets even harder to preserve the unique history and culture of each region. By providing a `Kala Kendra’ for the artists and craftsmen of Neemrana Village, this project will look in depth at the problem of gradual erosion of the region’s intangible heritage. By analyzing the present-day challenges encountered by regional identity around the world, this project will look into the elements that form the heritage of Neemrana Village, as well as the persistent problems within the village that are causing the extinction of these elements. To preserve the culture of Neemrana, this thesis looks at the past attempts made around the world to address similar issues. Also, by evaluating the traditional and vernacular architecture of the village, as well as the arts and crafts of Neemrana, this project will use its essence to propose an adequate solution to preserve its heritage without causing hindrance to the village’s growth and development. Through blending methods of education and exhibition, a better typology will arise to more effectively serve the community of Neemrana Village. Such a project requires a revised understanding of the art and architecture and their maker’s process, and context. This new model will return art to the public to validate its importance as a process and not just the final product.

Committee:

Elizabeth Riorden|, M.Arch. (Committee Chair); Aarati Kanekar, Ph.D. (Committee Member)

Subjects:

Architecture

Keywords:

heritage;culture;artists;craftsmen;Neemrana Village;Kala Kendra

Romero, VeronicaComputational Measurement of Social Communication Dynamics in Children with Autism Spectrum Disorder
PhD, University of Cincinnati, 2017, Arts and Sciences: Psychology
Adolescents with Autism Spectrum Disorder (ASD) exhibit impairments in social interactions that last throughout their lives and at the core of these impairments are social communication deficits. An ever growing body of literature has shown that language and interpersonal motor coordination are intertwined and both very important to achieve successful social interactions. Understanding where this relationship breaks down in children with ASD has the potential to provide great insights into the social communication deficits observed in the disorder. The current project used a standard clinical assessment tool of social communication (ADOS-2) to explore and determine the time-evolving semantics present in verbal communications, how these measures were related to frequently-used qualitative measures and how the verbal and nonverbal components of communication were related to each other in children with and without ASD. More specifically, the project: (1) quantified verbal conversational dynamics through the use of Discursis and categorical recurrence quantification analysis; (2) quantified the nonverbal aspects of conversational exchanges (by looking at bodily movement dynamics) using pixel change time-series extraction and continuous recurrence quantification analysis; and finally (3) determined the relationship between verbal and nonverbal aspects exhibited during the conversational exchanges and traditional measures used to quantify ASD severity through the use of correlational methods. Experiment 1 consisted of children between the ages of 6 and 10 years, all previously diagnosed with ASD to test the differences (or lack thereof) of behaviors exhibited by the children in two kinds of conversations (free flow vs. structured) given that children with ASD are seen as having difficulties adjusting behavior to suit various contexts. Experiment 2 had older participants (between 10-15 years of age) as well as typically developing children who were recruited to complete the ADOS-2 and serve as an age-matched control group, so that the expected social behaviors could be compared to those exhibited by children previously diagnosed with ASD. Differences were found between some verbal behaviors exhibited by children with and without ASD. These differences were not only confined to the pragmatics of language, but also to semantic and lexical components. Nonverbal behaviors exhibited during conversations involving children with ASD differed from those involving typically developing child. More importantly, both verbal and nonverbal dynamical measures were related to the traditional measures used to diagnose ASD and measure symptom severity. As a result, the dynamical measures explored here have the potential to improve diagnostic and therapeutic interventions in the future. However, some of the results observed were inconclusive due to low power. A larger sample size and a greater variety of test administrators and age groups should be explored in the future.

Committee:

Michael Richardson, Ph.D. (Committee Chair); Amie Duncan (Committee Member); Paula Fitzpatrick, Ph.D. (Committee Member); Michael Riley, Ph.D. (Committee Member); Paula Silva, Ph.D. (Committee Member)

Subjects:

Psychology

Keywords:

Autism Spectrum Disorder;Social communication dynamics;Discursis;Recurrence quantification analysis;ADOS-2

Xie, JiajingAn Architectural Rendition of Shadow Puppetry: a Translation from Shadow Puppetry to Architecture Through Movement
MARCH, University of Cincinnati, 2017, Design, Architecture, Art and Planning: Architecture
Shadow puppetry is an ancient performing art. It uses silhouette figures that are manipulated to create the illusion of moving images in front of an illuminated screen. The characteristics of the movement in shadow puppetry include the rhythm of disappearing and reappearing; blurriness and darkness of shadows, which create dramatic effects. The analysis of movement in shadow puppetry provides an analogy from which design principles can be drawn. Through the exploration of the choreography of movement and circulation as well as a variety of translucent materials, the thesis offers an enriching experience of light, shadow, space and time. The design of a visitor resort for a bamboo park in a Chinese country, and a center for the exploration and performance of shadow puppetry, will be the vehicle for the exploration of the movement of shadows. The design uses specific devices analogous to shadow puppetry. Through the movement of shadows, the dramatic architectural promenade inspires a curiosity and leads the occupants to question their surroundings, just like watching a shadow puppetry show.

Committee:

Aarati Kanekar, Ph.D. (Committee Chair); Christoph Klemmt, A.A. Dipl. (Committee Member)

Subjects:

Architecture

Keywords:

shadow puppetry;movement;experience;space and time;light and shadow;architecture

Chávez Wulsin, AynaraGlucocorticoid Mechanisms of Epileptogenesis and Comorbid Emotional Dysregulation
PhD, University of Cincinnati, 2017, Medicine: Neuroscience/Medical Science Scholars Interdisciplinary
Dysregulation of the hypothalamo-pituitary-adrenocortical (HPA) axis is common in temporal lobe epilepsy. It is characterized by excess glucocorticoid signaling, a process that is similar to what is seen in a subset of patients with major depression. Although activation of the glucocorticoid receptor (GR) is essential for survival, exposure to excess glucocorticoids can lead to disruption of key neuronal circuits, particularly in the hippocampus. This disruption may compromise physiological and behavioral adaptations to stress. It is well documented that chronic exposure to excess corticosterone in rodents may be linked to the development of depressive and anxiety-like phenotypes. These effects can be blocked by treatment with GR antagonists. In addition to modulating behavior, glucocorticoids have been shown to exhibit pro-convulsant effects in several animal models of epilepsy. Thus hyperactivity of the HPA axis may be a common pathological mechanism that may help explain the high incidence of psychopathologies in epilepsy. The underlying mechanisms and long-term consequences of HPA axis hyperactivity in epilepsy are currently unknown. Exposure to elevated glucocorticoids may be particularly deleterious in epilepsy, as it may exacerbate hippocampal pathologies, increase brain excitability (pro-convulsant) and raise susceptibility for the development of depressive and anxiety-like phenotypes. These data support a role for GR in epileptogenesis. The studies presented in this dissertation aimed at testing our underlying hypothesis, that chronic hyper-activation of GR occurs early in the disease and contributes to the comorbid development of epilepsy and depression/anxiety-like phenotypes. In chapter 2, I used the pilocarpine induced status epilepticus (SE) mouse model of TLE to characterize changes in stress-regulatory circuitry and emotional behavior associated with TLE. These studies demonstrate that SE results in hypersecretion of baseline glucocorticoids. This elevation persists through the chronic period of epilepsy and is accompanied by increased glucocorticoid secretion in response to acute stress. Importantly, epilepsy generates profound impairments in the recruitment of forebrain circuits that are key in regulating stress inhibition and emotional reactivity. In chapter 3 I utilized the glucocorticoid antagonist RU486 to block glucocorticoid receptor signaling in the hours following SE. Glucocorticoid blockade successfully normalizes corticosterone secretion and reduces hippocampal pathologies associated with epileptogenesis, including reduction of mossy cell loss and decreased ectopic placement of post-SE generated cells. These findings support a role for GR in epileptogenesis. In chapter 4 we utilized a newly-develop glucocorticoid receptor modulator C108297 to test whether modulation rather than complete blockade has beneficial potential in epileptogenesis. Results from this study demonstrate a disease modifying effect of C108297 in the hippocampus and promising reduction in seizure severity. The studies conducted as a part of this dissertation research may prove critical for understanding mechanisms of epileptogensis, and suggest the potential of GR as a therapeutic target for the treatment of epilepsy and its comorbidities.

Committee:

Matia Solomon, Ph.D. (Committee Chair); Steve Danzer, Ph.D. (Committee Member); James Herman, Ph.D. (Committee Member); Louis Muglia, M.D. (Committee Member); Michael Privitera, M.D. (Committee Member)

Subjects:

Neurology

Keywords:

glucocorticoids;temporal lobe epilepsy;status epilepticus;C108297;Mifepristone;mice

Chaton, Catherine TMetal Binding Specificity and N-terminal Function of the Staphylococcal Biofilm Protein Aap
PhD, University of Cincinnati, 2017, Medicine: Molecular Genetics, Biochemistry, & Microbiology
Staphylococcus epidermidis, a typical human commensal bacterium, is a serious contributor to hospital-acquired infections (HAI) when accidently introduced to surgical sites and in the immunocompromised. The majority of S. epidermidis HAIs involve the formation of macrocellular structures called biofilms. These specialized surface-adherent colonies confer resistance to antibiotics and shield the bacteria from recognition by the host immune response. The accumulation-associated protein (Aap) is a multifunctional cell-wall protein which mediates intercellular adhesion through metal-dependent self-assembly. In addition, it contains elements for the recognition and attachment to host-cell surface features. The N-terminus of Aap begins with a sequence of short 16-residue `A-repeats’ which are followed by a globular lectin domain. The C-terminal half contains 5-17 tandem copies of B-repeat domains, which, once proteolytically processed from the N-terminal domains, are capable of forming anti-parallel, intertwined protein bridges. This assembly requires coordination to divalent cations through each B-repeat’s G5 domain. Previously solved crystal structures of a minimal B-repeat construct (Brpt1.5) have characterized a zinc-dependent dimer. This work describes novel research into the specificity and selectivity of the metal-coordination and N-terminal regulation of Aap by the A-repeats. Cu2+ has been found to be capable of inducing assembly using the same coordinating residues as Zn2+. Longer constructs of B-repeat (Brpt5.5) with an increased number of G5 domains require lower metal concentrations for assembly. The boundary between monomer to dimer transition also narrows, suggesting cooperativity between the G5 domains. By responding to increasing local concentrations of Zn2+ and Cu2+, divalent metals both implicated in host immunity, Aap can act as an environmental sensor. However, Aap is only capable of assembly once the A-repeats have been proteolytically processed from the tandem B superdomain. Interactions between the A-repeats and the unique sequence of the first G5 domain act to block B-repeat assembly until processing. The N-terminal residues of the A-repeats fold back in a hairpin structure, both regulating Aap assembly and positioning the globular lectin domain in an optimal orientation for host-cell attachment. While the specific targets have not yet been identified, initial structural analysis indicates it shares many characteristics with other lectin domains found in staphylococcal virulence factors. Better understanding of Aap’s N-terminal regulation and domain function reveals novel targets for the development of monoclonal antibody and small-molecule inhibitor based therapeutics.

Committee:

Andrew Herr, PhD (Committee Chair); George Deepe, PhD (Committee Member); Rhett Kovall, PhD (Committee Member); William Miller, PhD (Committee Member); George Stan, PhD (Committee Member)

Subjects:

Biophysics; Medicine

Keywords:

accumulation associated protein;Staphylococcus epidermidis;biofilm;hospital-acquired infection;macromolecular assembly;analytical ultracentrifugation

Bhave, Chittatosh CA Computational Study of the Heat Transfer Characteristics of Offset-Strip Fin Cores
MS, University of Cincinnati, 2017, Engineering and Applied Science: Mechanical Engineering
Enhanced extended surfaces such as offset-strip fins (OSF) are effective in increasing the area density as well as altering the convective flow behavior to provide higher heat transfer coefficient in compact heat exchangers. This is achieved by periodic disruption and reattachment of the new thin boundary layer on the fin plate of each offset-strip fin module. The heat transfer characteristics and the flow physics inside the OSF cores is revisited in this computational analysis for laminar air flows (Pr = 0.7) and their performance is compared with plain fins. A simplified model of thin fins is used to study the effect of fin geometric parameters, viz., offset-fin length l, fin separation s and fin thickness t. The parametric variation is restricted to the practical range of fin density (8 fpi to 22.6 fpi) and low blockage ratio to the flow (<20%) while systematically increasing the offset-fin length (1 < l/s < 35). The results show that the short offset-fin length provides higher heat transfer enhancement compared to the plain rectangular fins, while the fin thickness and fin separation show negligible improvement in heat transfer rate for a constant offset-fin length ratio (l/s). The offset-fin effect diminishes as Reynolds number decreases or the offset-fin length becomes very large, as the OSF performance asymptotically approaches towards that of a plain rectangular fin. The OSF cores are shown to reduce the heat transfer surface area by 30% - 50% while keeping a constant pressure drop as that for a plain rectangular fin. A practical case with short offset-fin length (~ 3mm) having a squarer cross-section fins (s/h > 0.5) with intermediate to low fin density (8 fpi to 12 fpi) provides smaller pressure drop gradients as well as larger heat transfer enhancement capacity for a constant heat duty application.

Committee:

Milind Jog, Ph.D. (Committee Chair); Je-Hyeong Bahk, Ph.D. (Committee Member); Raj Manglik, Ph.D. (Committee Member)

Subjects:

Mechanical Engineering

Keywords:

Heat Transfer;Offset-Strip Fin

Riffle, StephenMulticellular Tumor Spheroids as a Model to Study Tumor Cell Adaptations within a Hypoxic Environment
PhD, University of Cincinnati, 2017, Medicine: Molecular and Developmental Biology
Hypoxia is a common feature in solid tumors which facilitates the development of therapeutically resistant and aggressive tumor phenotypes. As oxygen levels decrease, cellular metabolism and energy production becomes impaired and the potential for catastrophic DNA damage increases. To prevent cell death, tumor cells undergo several adaptations which prevent depletion of nutrients, minimize DNA damage, and re-acquire blood flow. The mechanisms behind tumor cell survival during hypoxia are poorly understood. Tumor neovascularization is a critical step in facilitating continued tumor growth. Accordingly anti- angiogenic therapy has shown significant success in preventing progression free survival however increased tumor aggression and alternative neovascularization methods result in decreased overall patient survival. Vasculogenic mimicry is one such proposed method of alternative neovascularization wherein tumor cells transdifferentiate and form de novo vascular structures. Although there are indications that this process is driven by hypoxia and is linked to aggressive tumor phenotypes, there is little known about the signaling pathways inducing such an adaptation. Evidence from in vitro studies suggest that activation of DNA damage repair proteins in proliferating hypoxic cells is critical for sustained proliferation and cell viability thus implicating repair proteins as potential therapeutic targets. In vivo studies demonstrating a correlation between hypoxia and the formation of γ-H2AX further support this potential. The evaluation of such therapeutic targets is limited by the inability of in vitro monolayer culture models to recreate therapeutically relevant aspects of a 3D in vivo tumor. Multicellular tumor spheroids (MCTS) represent a 3D in vitro culture model in which tumor cell adaptations to the hypoxic tumor microenvironment are recreated. These include the accumulation of metabolic waste, appearance of nutrient gradients, and development of proliferative heterogeneity. MCTS have been used extensively to characterize radiation resistance in a hypoxic environment.We set out to describe the relationship between proliferation, hypoxia, DNA damage repair, and vasculogenic mimicry using the MCTS model. Using MCTS, we demonstrate for the first time the activation of DNA damage repair proteins in hypoxic proliferating cells residing within the MCTS core and further show the ability to study the efficacy of therapeutics targeting this cell population. Although metabolic adaptations and DNA damage repair protein activation are observed in this model, MCTS formed using the liquid overlay method do not experience vasculogenic mimicry. The results presented herein advances the field of tumor biology by validating the utility of MCTS as a model system in which clinically relevant features of a tumor can be recreated and studied. Furthermore our studies identify the Eyes Absent tyrosine phosphatase as a therapeutic target whose activity regulates DNA damage repair under both normoxic and hypoxic conditions and may be involved in vasculogenic mimicry.

Committee:

Rashmi Hegde, Ph.D. (Committee Chair); Zelia Correa, M.D. Ph.D. (Committee Member); Richard Lang, Ph.D. (Committee Member); Qing Richard Lu, Ph.D. (Committee Member); Kathryn Wikenheiser-Brokamp, M.D. Ph.D. (Committee Member)

Subjects:

Cellular Biology

Keywords:

Multicellular Tumor Spheroids;DNA damage repair;Eyes Absent;Hypoxia;Cancer;Ewing Sarcoma

Liu, ZhouyangHeterogeneous Catalytic Elemental Mercury Oxidation in Coal Combustion Flue Gas
PhD, University of Cincinnati, 2017, Engineering and Applied Science: Chemical Engineering
The new Mercury and Air Toxics Standards issued by US EPA require the reduction of mercury emissions from coal-fired power plants by 90% starting from 2016. Oxidizing elemental mercury using the HCl that exists in the flue gas or additional halogen and catalysts, followed by oxidized mercury capture in the wet Flue Gas Desulfurizer (FGD), is a viable option for mercury removal in coal-fired power plants. The aim of this study is to develop effective and reliable mercury oxidation catalysts, advance the mechanistic understandings of heterogeneous mercury oxidation, and obtain information on heterogeneous mercury oxidation kinetics. CuCl2 supported on ?-Al2O3 showed excellent Hg(0) oxidation performance and SO2 resistance at 140 °C. After extensive characterizations of the CuCl2/?-Al2O3 catalyst, the existence of multiple copper species was identified. It was found that CuCl2 forms inert copper aluminate on the surface of ?-Al2O3 at lower loadings. At higher loadings, CuCl2 exists in a highly dispersed amorphous form that is active for Hg(0) oxidation by working as a redox catalyst. The CuCl2/?-Al2O3 catalyst with high loadings has the potential to be used as a low temperature Hg(0) oxidation catalyst. RuO2 catalyst was found to be an excellent Hg(0) oxidation catalyst. When rutile TiO2 was used as the catalyst support, RuO2 formed well dispersed nano-layers due to the very similar crystal structures of RuO2 and rutile TiO2, giving higher Hg(0) oxidation activity over anatase TiO2 support. The RuO2/rutile TiO2 catalyst showed good Hg(0) oxidation performance under sub-bituminous and lignite coal simulated flue gas conditions with low concentration of HCl or HBr gas. It also showed excellent resistance to SO2. The RuO2/rutile TiO2 catalyst can be used at the tail end section of the SCR unit for Hg(0) oxidation. Linear combination fitting of the X-ray Absorption Near Edge Spectroscopy (XANES) was used to quantify oxidized mercury species over RuO2/TiO2 and SCR catalysts under different simulated flue gas conditions. In the absence of halogen gas, elemental mercury can react with sulfur that is contained in both the RuO2/TiO2 and SCR catalysts to form HgS and HgSO4. In the presence of HCl or HBr gas, HgCl2 or HgBr2 is the main oxidized mercury species. When both HCl and HBr gases are present, HgBr2 is the preferred oxidation product and no HgCl2 can be found. Other simulated flue gas components such as NO, NH3, SO2 and CO2 do not have significant effect on oxidized mercury speciation when halogen gas is present. Mechanistic and kinetic studies of the heterogeneous oxidation of Hg(0) by HCl gas over a RuO2/rutile TiO2 catalyst were conducted. The experimental evidence of HCl adsorption was obtained using in-situ Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS). Based on this result, a steady-state kinetic study was conducted to determine an intrinsic reaction kinetic expression for Hg(0) oxidation over the catalyst under HCl, NH3 and SO2 gases for the first time. The kinetic expression obtained could reasonably predict the Hg(0) oxidation performance under the competitive adsorption of NH3 and SO2 gases.

Committee:

Joo Youp Lee, Ph.D. (Committee Chair); Anastasios Angelopoulos, Ph.D. (Committee Member); William Connick, Ph.D. (Committee Member); Peter Panagiotis Smirniotis, Ph.D. (Committee Member)

Subjects:

Chemical Engineering

Keywords:

heterogeneous catalysis;mercury removal;kinetic;mechanism;characterization;ruthenium oxide

Ondja'a, BertinUniversity Social Responsibility: Achieving Human and Social Development in Cameroon
PhD, University of Cincinnati, 2017, Education, Criminal Justice, and Human Services: Educational Studies
As place-based institutions, higher education institutions (HEIs) have the intellectual capacity and resources, both private and public, to transform communities facing human and social development issues. As such, HEIs must revisit their role/contribution and recognize their social responsibility to remain relevant in today’s society. Indeed, social responsibility requires new community research and revitalization, job creation, teaching, and innovation. This study explores the role/contribution of Cameroonian HEIs in addressing human and social development issues through a qualitative case study of the University of Douala. More specifically, this study explored how the university advances development agendas through the use of interviews and a photovoice project, examining historical, social, and political events and practices that frame the role/contribution of HEIs in Cameroon. The findings suggest that HEIs are an important asset for the community and make significant educational, social, environmental, economic, and cultural contributions to the community. Specifically, the findings indicate the contribution of the University of Douala in the following areas: service, innovation, advocacy, leadership, and capacity building. Consequently, HEIs can help to advance human and social development. However, their contribution in these areas remains underutilized. Also, stakeholder engagement and lack of funding emerge as elements that downplay the contribution of the University of Douala in human and social development issues. Adaptive solutions for complex issues should consider the potential contribution of HEIs. This study concludes with recommendations and implications that aim to educate different stakeholders and practitioners on how HEIs can maximize their potential. En tant qu'institutions operant dans les territoires, les etablissements d'enseignement superieur possedent des capacites intellectuelles et materielles pouvant transformer les communautes confrontees aux problemes de developpement humain et social. En tant que tel, ils doivent regulierement actualiser leur engagement et mission envers la societe pour rester pertinent au regard d’un environnement instable et imprevisible. En tant que potentiel levier du developpement durable, ils doivent adopter la responsabilite sociale. En effet, la responsabilite sociale des universites (RSU) doit etre considerer comme etant un precurseur du reveil communautaire, creation d’emplois, developpement economique et durable, et innovation technologique. La presente etude explore l’apport des universites au developpement humain et social dans un contexte Camerounais. Une etude de cas qualitative realisee a l’Universite de Douala par le biais des entretiens et un projet photovoice et en tenant compte de l’environnement historique, social et politique dans lequel l’enseignement superieur s’est forge, cette etude demontre la capacite des universites a accompagner l’Etat dans ses multiples missions. Cependant, ces atouts restent largement sous-exploites. Dans le cas precis de l’Universite de Douala, le manque d’engouement des parties prenantes, l’organisation institutionnelle et l’absence de financement apparaissent comme etant des elements qui minimisent la contribution de l'Universite de Douala aux problemes de developpement humain et social dans son milieu. Projeter des solutions adaptees aux problemes complexes devrait tenir compte du potentielle des etablissements de l’enseignement superieur. Cette etude se termine par des prescriptions et des incidences qui visent a sensibiliser les agents de developpement sur la facon dont ces etablissements peuvent maximiser leur contribution au developpement humain et social.

Committee:

Constance Kendall Theado, Ph.D. (Committee Chair); Mary Brydon-Miller, Ph.D. (Committee Member); David Edelman, Ph.D. (Committee Member); Joseph Takougang, Ph.D. (Committee Member)

Subjects:

Higher Education

Keywords:

University Social Responsibility;Photovoice;Regional Development;University Engagement;Human and Social Development;Growth Pole

Nickum, Elisa AAnalysis of Regulated Drugs Using Chromatographic and Spectrophotometric Techniques Coupled with Spectroscopy An Orthogonal Approach to Protecting Public Health
PhD, University of Cincinnati, 2017, Arts and Sciences: Chemistry
A considerable number of dietary supplements suspected of containing phosphodiesterase-5 (PDE-5) inhibitors and substituted phenethylamines have been analyzed by the U.S. Food and Drug Administration. Often these samples are found to contain the active pharmaceutical ingredients (API) such as sildenafil or phentermine, and in many cases, products contain multiple PDE-5 inhibitors or substituted phenethylamines. In an analytical setting, it is important to confirm the presence of any API with two or more independent methods, and this requirement can often put undo strain on a laboratory. The development and use of methods that inherently contain two unique identification techniques is preferred, and the creation and validation of three of those methods is outlined here. First, direct deposit Fourier transform infrared detection and mass spectrometric detection (GC/FT-IR/MS) is used to identify PDE-5 inhibitors. Generally, GC/MS is not generally used for this category of drugs due to low volatility; PDE-5 inhibitors often co-elute and can produce non-specific electron ionization fragmentation patterns. In contrast, FT-IR has been proven to be more selective for identifying PDE-5 inhibitors, but is generally not as sensitive as spectrometric techniques. However, it has been shown that each technique can compensate for the other, which allows a wider range of usability. Using this combined technique can save time and resources while still delivering a high level of certainty in identification by providing results from two scientifically uncorrelated techniques. Multiple reference standards were utilized for method validation, including determination of linearity, dynamic range, and limit of detection. Second, a single HPLC-UV method has been developed for the determination of PDE-5 inhibitors and related analogs in pharmaceutical dosage forms and dietary supplement products. Using this protocol, 14 PDE-5 inhibitor compounds can be separated and determined in a single analysis. Multiple reference standards were utilized for method validation, including determination of linearity, dynamic range, injection precision, limits of detection and quantitation, accuracy and precision. It was also demonstrated that, in cases where a standard for a specific analog is not readily available, another reference standard can be used to approximate the level of analog present, based on similarities in their chemical structures and absorbance spectra. Third, an HPLC-UV method has been developed for the determination of substituted phenethylamines in pre-workout dietary supplements. The number of these products on the market has greatly increased in recent years, and the labeled ingredients did not seem to account for amazing energy and euphoria experienced by users. Using GC-MS analysis, methamphetamine-like compounds are often detected but can be difficult to identify; as standards are not always available for comparison. To characterize any new analog, it is necessary to separate it from the matrix using an acid-base extraction, followed by HPLC-UV fraction collection and characterization using HRAM-MS and nuclear magnetic resonance. Recently, these techniques were used to characterize N-ethyl-a-ethylphenethylamine in a powdered drink supplement. Using this HPLC-UV method, five substituted phenethylamines can be separated and determined in a single analysis. Multiple reference standards were utilized for method validation, including determination of linearity, dynamic range and injection precision.

Committee:

Peng Zhang, Ph.D. (Committee Chair); Anna Gudmundsdottir, Ph.D. (Committee Member); Laura Sagle, Ph.D. (Committee Member)

Subjects:

Chemistry

Keywords:

PDE 5 Inhibitor;GC MS;FT IR;HPLC UV;GC FT IR MS;Phenethylamine

Locke, KellieUnknown Encounters: Surrealist Thought Examined for Provoking Self-Reflection in Architecture
MARCH, University of Cincinnati, 2017, Design, Architecture, Art and Planning: Architecture
The creation of architecture is often approached as a social act: many buildings are created for public consumption, and many architects believe the users’ experiences (and by association the architecture itself) are enriched through encountering public spaces and opportunities to interact with other users or with the public at large. Moments are identified within the design where potentially disparate paths will cross and the people on them will acknowledge and understand each other. This investigation argues that the same architectural tools used to engineer these moments of collision may also be employed differently to be productively disruptive of the user’s journey, creating moments of mental solitude. To that end, this thesis explores the architecture of self-reflection, solitude, and self-understanding. Rather than identifying ways to encourage interaction with others, this is an examination of how architecture might suggest that users look inward. To create architectural experiences of this nature, inhabitants’ visual and spacial perception are challenged through manipulation of form and space. In the perceptual vacuum that is created when the user’s idea of reality is denied, the inhabited space is, in a sense, Surreal. This investigation proposes that when we are unsure of the space we inhabit, and the Surreal dominates, we are given a moment to look inward. In this way, the spacial constructions informed by surrealist thought can promote meditation and reflection. Building on surrealist intentions, themes of the surreal may be applied to architecture to fulfill similar architectural intentions of this thesis: questioning the true nature of our environment to promote self-reflection. Through an iterative case study exercise, architectural form is manipulated using strategies informed by surrealist ideology to challenge the user’s perception of reality, providing an opportunity to reflect.

Committee:

Christoph Klemmt, A.A. Dipl. (Committee Chair); Aarati Kanekar, Ph.D. (Committee Member)

Subjects:

Architecture

Keywords:

surrealism;perception;meditation;reflection

Bdaiwi, AbdullahCategorizing Cortical Dysplasia Lesions for Surgical Outcome Using Network Functional Connectivity
MS, University of Cincinnati, 2017, Arts and Sciences: Physics
Lesion-symptom mapping is a powerful and broadly applicable approach that is used for linking neurological symptoms to specific brain regions. Traditionally, it involves identifying overlap in lesion location across patients with similar symptoms. This approach has limitations when symptoms do not localize to a single region or when lesions do not tend to overlap. In this thesis, we show that we can expand the traditional approach of lesion mapping to incorporate network effects into symptom localization without the need for specialized neuroimaging of patients. Our approach involves assessing the functional connectivity of each lesion volume with the rest of the typical healthy brain using a database of healthy pediatric brain imaging data (C-MIND), available at CCHMC. Our study included 24 subjects that had cortical dysplasia lesions and underwent surgery for seizures that did not respond to drug therapy. We tested our approach using healthy brain imaging data across all ages (2-18 years old) and using age & gender specific groupings of data. The analysis sought categorization of lesion connectivity based on five subject characteristics: gender, cortical dysplasia pathology, epilepsy syndrome, scalp EEG pattern and surgical outcome. Our primary analysis focused on surgical outcome. The results showed that there are some substantial connectivity differences in the outcome analysis. Lesions with stronger connectivity to default mode and attention/motor networks tended to result in poorer surgical outcomes. This result could be expanded with a larger set of data with the ultimate goal of allowing examination of lesions of cortical dysplasia patients and predicting their seizure outcomes.

Committee:

Mark Difrancesco, Ph.D. (Committee Chair); Darren Kadis (Committee Member); Rostislav Serota, Ph.D. (Committee Member); L. C. R. Wijewardhana, Ph.D. (Committee Member)

Subjects:

Physics

Huffman, AshleyDiscovery of Movement: An Exploration of Physical Activity through Architecture
MARCH, University of Cincinnati, 2017, Design, Architecture, Art and Planning: Architecture
Insufficient physical activity is a key factor in cardiovascular diseases, cancers, and diabetes. The increase in physical inactivity is a large contributor to the worldwide obesity epidemic. Social changes and economic growth increased the sedentary activities like watching television, using the computer, and playing video games. Promoting activities through daily living, walking, using the stairs, can increase the total energy expenditure that can help in maintaining energy balance or even losing weight. Architecture can be more than a space for people to occupy, it can become the foundation for which people move, thus exploring the role of how the built environment can help facilitate in a healthier lifestyle. Focusing on the impacts of public health, the built environment can help facilitate an active spaces that will encourage people to move. By incentivizing a series of towers that will allow people of all ages to passively engage in exercise without realizing it, through walking and climbing stairs, they reap the benefits of improving their overall health.

Committee:

Elizabeth Riorden, M.Arch. (Committee Chair); Udo Greinacher, M.Arch. (Committee Member)

Subjects:

Architecture

Keywords:

physical inactivity;obesity;health;stairs

Godfrey, Lisa MSexual Agreements in Young Male Same-Sex Couples: Associations with Relationship Quality and Stability
MA, University of Cincinnati, 2018, Arts and Sciences: Psychology
In this study, I investigated whether sexual agreements (monogamous, non-monogamous, or no sexual agreement) were associated with relationship quality and stability in a sample of young men who have sex with men (YMSM). A sample of 312 YMSM in same-sex relationships reported on their sexual agreement and indices of relationship quality (satisfaction, trust, and commitment) at baseline, along with their relationship status (still together or broken up) at 6- and 12-month follow-up. One-way ANCOVAs controlling for age indicated no differences by sexual agreement in concurrent trust, but YMSM with monogamous agreements reported higher satisfaction and commitment than YMSM with non-monogamous agreements and those with no sexual agreement. An interval-censored survival analysis revealed no differences in the occurrence of break-up at 6- or 12-month follow-up by sexual agreement. However, post-hoc analyses indicated that this analysis was underpowered, and indirect effect analyses revealed that having a monogamous agreement (vs. a non-monogamous agreement or no agreement) was indirectly associated with higher relationship stability through relationship commitment. These findings demonstrate that YMSM with monogamous agreements may have higher relationship quality at early relationship stages, and that monogamous agreements may be a protective factor against break-up through the mechanism of relationship commitment.

Committee:

Sarah Whitton, Ph.D. (Committee Chair); Jennifer Brown, Ph.D. (Committee Member); Kristen Jastrowski Mano, Ph.D. (Committee Member)

Subjects:

Psychology

Keywords:

Sexual agreements;Relationship quality;Relationship stability;Young men who have sex with men;Monogamy;Male same-sex relationships

Venkatesh, ChandrasekarAdvanced Weather Monitoring for a Cable Stayed Bridge
PhD, University of Cincinnati, 2018, Engineering and Applied Science: Electrical Engineering
In the northern United States, Canada, and many northern European countries, snow and ice pose serious hazards to motorists. Potential traffic disruptions caused by ice and snow are challenges faced by transportation agencies. Successful winter maintenance involves the selection and application of the most optimum strategy, over optimum time intervals. The risk associated with operating the bridges during winter emergencies varies depending on the size of the structure, the material of the stays, volume of average daily traffic, geographical location, nature of terrain and surroundings etc. The `Dashboard’, a monitoring system designed to help the bridge maintenance and operation personnel was developed at University of Cincinnati Infrastructure Institute. This was implemented at the Veterans Glass City Skyway Bridge in Toledo, Ohio. This system was also extended to the Port Mann Bridge in Vancouver, Canada. The aim of this research is to come up with an advanced monitoring system which will help the bridge management team make control actions during winter emergencies on the VGCS and Port Mann bridges. The current monitoring system gives information on the status of ice accumulation/ snow accretion or shedding based on last one hour’s weather data. This dissertation focuses on adding intelligence to the existing system through addition of sensors, identifying patterns in events, adding cost-benefit analysis and incorporating forecast parameters, while also extending the system to other bridges and structures. In essence a new, more intelligent monitor designed to make the control decisions easier and have all necessary information to make such decisions in one place will be invaluable to the officials in the transportation departments.

Committee:

Arthur Helmicki, Ph.D. (Committee Chair); Victor Hunt, Ph.D. (Committee Member); Ali Minai, Ph.D. (Committee Member); Douglas Nims, Ph.D. (Committee Member); William Wee, Ph.D. (Committee Member)

Subjects:

Engineering

Keywords:

Dashboard;Weather monitoring;Bridge operations;Snow emergency;Ice emergency;Weather forecast

Tobe, RachelThe Chimera
MARCH, University of Cincinnati, 2017, Design, Architecture, Art and Planning: Architecture
My goal is to design a home for user-directed change. Studies of kinetic architecture have tackled this in different ways, often not overlapping multiple methods for the sake of design simplicity. I intend to merge three usually separate scales of kinetic architecture (the scale of the whole building, the scale of the exterior skin, and the scale of the interior program) to create a completely new flexible housing option. This will provide the user with the ability to control their location, the interior of their home, and the connection they have to their site. With this new design type, I will offer informed predictions for the ramifications and impacts this design would have on its user and on whole communities.

Committee:

Udo Greinacher, M.Arch. (Committee Chair); Aarati Kanekar, Ph.D. (Committee Member)

Subjects:

Architecture

Keywords:

Kinetic Architecture;Residential Architecture;Flexible Housing;Mobile Architecture;Flexible Interiors;Tiny House

Estrada, Christina MThe Impact of Obesity and Estrogen on the Brain and Metabolic Function in Female Rats
PhD, University of Cincinnati, 2018, Arts and Sciences: Psychology
Obesity is a national health concern, and obese menopausal women are at greater risk for diabetes and cardiovascular disease than their male counterparts. Notably, obesity is comorbid with anxiety and depression, particularly in women, suggesting shared hormonal or brain networks linking mood and metabolic disorders. Estrogens, particularly 17β-estradiol (E2), maintain energy and metabolic homeostasis by offsetting excess weight gain and obesity-related consequences in women. E2 is circulated through the bloodstream to various organs including the brain, e.g. hypothalamus and medial amygdala (MeA) which regulate mood and metabolism. Unfortunately, being obese may disrupt the protective actions of E2 and ultimately dampen the brain’s ability to effectively regulate energy homeostasis. Thus, it is important to understand how obesity impacts brain function in females and whether these potential effects are reversible or permanent. This research includes a series of experiments conducted in adult female rats in order to (1) determine the impact of high fat diet (HFD) on metabolic and reproductive function, and estrogen receptor (ER&alpha) expression in metabolic-regulatory brain regions in ovarian-intact rats, to mimic a pre-menopausal state; (2) determine whether peripheral E2 reverses obesity and obesity-related consequences in adult-HFD ovariectomized (OVX) female rats, designed to mimic a menopausal state; (3) determine whether central E2 administration in the MeA via stereotaxic surgery, prevents obesity and obesity-related consequences in “menopausal” rats. Lastly, we determined the impact of E2 in the MeA on neuronal activity in stress and metabolic-regulatory brain regions. High-fat diet increased body weight and body fat, decreased muscle mass, impaired glucose tolerance, and induced irregular estrous cycles in young adult ovarian-intact female rats. These findings suggest that diet-induced obesity produces a metabolic and reproductive profile that is akin to aged females. Despite marked changes in metabolic function, HFD did not alter ERα expression in brain regions involved in energy balance. Obese OVX rats fed a HFD had increased body weight and fat mass, decreased energy expenditure, and higher glucose levels, compared with low-fat fed females. However, E2 treatment in obese OVX-HFD females did not improve glucose tolerance or decrease abdominal fat. This suggests that some benefits of E2 lessen with obesity or following a significant time after E2 loss. E2 in the MeA also prevented excess food intake and body weight gain, increased visceral adiposity, and glucose intolerance that is typically seen in OVX rats. Finally, we found that E2 in the MeA decreased anxiety-like and depression-like behaviors in OVX rats, which may be due to its ability to modulate neuronal activity in mood and stress-regulatory brain regions. Our findings reveal that obesity induces an accelerated aging phenotype on body composition and reproductive function in young rats. Though E2 is typically beneficial in women, obesity and or a significant time after E2 loss (e.g. menopause) may weaken the metabolic and reproductive benefits of E2. Most importantly, we demonstrate that the MeA mediates estrogenic effects on mood and energy homeostasis in females; which makes it a target region for treating the comorbidity of obesity, depression and or anxiety, particularly in women.

Committee:

Matia Solomon, Ph.D. (Committee Chair); James Herman, Ph.D. (Committee Member); Farrah Jacquez, Ph.D. (Committee Member); Quintino Mano, Ph.D. (Committee Member)

Subjects:

Behavioral Sciences

Keywords:

medial amygdala;estradiol;rodent;menopause;obesity;depression and anxiety

Whitehurst, Lauren MHome Game
MARCH, University of Cincinnati, 2018, Design, Architecture, Art and Planning: Architecture
The intent of this thesis is to define an approach to design a multi-unit housing project that will achieve a set of discrete goals aimed at democratizing architecture. Goals 1. Make architectural design more transparent. 2. Give power to the individual through knowledge and tools. 3. Involve and consider personal individuality. 4. Teach the masses about space and design.

Committee:

Vincent Sansalone, M.Arch. (Committee Chair); Michael McInturf, M.Arch. (Committee Member)

Subjects:

Architecture

Keywords:

home;architecture;teach;game;democratizing;knowledge

Fujiwara, HisakoCortical Morphology and Neuropsychological Performance in Idiopathic Childhood Epilepsy
PhD, University of Cincinnati, 2018, Medicine: Neuroscience/Medical Science Scholars Interdisciplinary
Approximately 50 million people worldwide live with epilepsy and making this disease one of the most common neurological diseases worldwide. About 0.6 – 2 % of children aged 0-17 years have active epilepsy. Childhood epilepsy affects children at different ages and in many different ways; some seizures in childhood are not associated with a definite cause. The most common type of idiopathic focal epilepsy syndrome is Childhood Epilepsy with Centrotemporal Spikes (CECTS). CECTS is age-dependent and self-limited. CECTS was previously considered a `benign’ epilepsy, because of the excellent seizure prognosis. However, there are increasing reports that children with CECTS exhibit various cognitive and behavioral problems. Recently, quantitative structural MRI analyses have shown that there is atypical cortical morphology in CECTS compared to typically developing children. However, the findings are often contradictory, which may be due to heterogenous study populations. Therefore, the aim of this study was to compare cortical thickness between drug-naive new onset CECTS patients and typically developing children, with careful inclusion criteria to promote homogeneity within groups and careful matching between. We also investigated the correlation with Processing Speed Index (PSI), which was significantly different between groups, and frequency of centrotemporal spikes (CTS) within regions of interest (ROIs). We did not find any cortical thickness differences between groups based on the whole brain analysis. We found a significant interaction between PSI and group in cortical thickness within the ROIs. There were positive correlations with PSI and cortical thickness in typically developing children, but no or negative correlation in CECTS. In addition, cortical thickness in right pars opercularis was thinner with higher frequency of right –sided CTS. These findings indicate that children with CECTS have atypical cortical features which may underlie poorer processing speed.

Committee:

Steve Danzer, Ph.D. (Committee Chair); Mekibib Altaye, Ph.D. (Committee Member); Christina Gross, Ph.D. (Committee Member); Darren Kadis, Ph.D. (Committee Member); Jeffrey Tenney, M.D. (Committee Member); Jennifer Vannest, Ph.D. (Committee Member)

Subjects:

Neurology

Keywords:

Childhood Epilepsy with Centrotemporal Spikes;Childhood Absence Epilepsy;Cortical Morphology;Neuropsychological Performance

Sivakumar, AnjanaContextualizing a Culture in the Diasporic Contemporary
MARCH, University of Cincinnati, 2018, Design, Architecture, Art and Planning: Architecture
We live in an increasingly diverse world, where diaspora is the norm and the exposure to immigrant cultures is a part of daily life. Immigrant groups rely on third places, as defined by Ray Oldenburg, to connect and collect the members of their cultural groups. This need exists among both the elder members of these communities and the younger members born here, in the United States. As they exist now, the third places for diasporic Indian communities raise two issues: they are exclusionary, and fall into two tropes: the co-opt and the transplant. These third places are often affiliated with religion, dividing members of cultural groups and rendering themselves as exclusionary spaces, fundamentally violating the basis of Oldenburg’s definition of the third place. Additionally, neither of these tropes adequately responds to the dual identities and values of Indians living in America today, begging the question: what is a cultural architecture that both respects its roots and responds to its current context? This thesis posits that an architecture that houses specific cultural activities must be performative, creating the conditions or environments that support the cultural behaviors and values that inform its design. Through an analysis and distillation of Indian culture, a corresponding student cultural center will be created to provide a place that is both culturally specific and inclusive for the Indian student organizations at the University of Texas at Austin to gather, celebrate, and share their culture with the larger student community.

Committee:

Aarati Kanekar, Ph.D. (Committee Chair); Michael McInturf, M.Arch. (Committee Member)

Subjects:

Architecture

Keywords:

performative architcture;Indian culture;Indian diaspora;student cultural center;architectural identity

Ariyabuddhiphongs, KrisSymmetry of Interpersonal Rhythmic Coordination: The Case of a Three-Person Drumming Task
PhD, University of Cincinnati, 2017, Arts and Sciences: Psychology
The mathematical theory of symmetry provides a framework to understand higher order structures of behavioral organization across various contexts; the same principle that explains the organization of quadruped gaits can also be applied to behavioral coordination in interpersonal contexts. The current studies examined how symmetries of perceptual coupling and social information influenced interpersonal coordination during a three-person drumming task. In Study 1, triads of participants performed a drumming task without explicit instructions to coordinate; each participant drummed to given metronome beats for 10 seconds and maintained his or her rhythm for the rest of the trial. Half of the 24 triads drummed at 60 bpm, and the other drummed at 45 bpm. Each triad performed the task under five auditory coupling conditions: the all-, rotation-, partial-, clamped-, and no-coupling conditions. During the task, participants could hear but not see each other’s drumming. The results showed that when coupling was present, the spontaneous coordination mode that emerged tended to be inphase. Regardless of drumming frequency, coordination in the all- and clamped-coupling conditions was more stable than in the partial-coupling conditions, indicating the effect of asymmetric coupling functions. In addition, period shifts were observed in the 45-bpm all-, rotation-, and clamped-coupling conditions. In Study 2, the minimal group paradigm was used to manipulate the symmetry of social identity among a triad. Fifteen triads were assigned to the heterogeneous condition, where two participants were in the minimal ingroup—the red group—and one in the minimal outgroup—the blue group. The other 14 triads were in the homogeneous condition (i.e., the control group) with all of them assigned to the red group. Beside the minimal group manipulation, there was no constraint on either visual or auditory information in Study 2. The participants first performed the drumming task without explicit instructions to coordinate (i.e., spontaneous coordination task) and, then, with explicit instructions to coordinate in a partial-inphase pattern (two participants inphase with each other and the third antiphase relate to the other two). The results showed that asymmetric minimal group identity had no effect on the spontaneous or explicit coordination. Plausible explanations for the null effects are discussed.

Committee:

Rachel Kallen, Ph.D. (Committee Chair); Michael Richardson, Ph.D. (Committee Member); Michael Riley, Ph.D. (Committee Member)

Subjects:

Psychology

Keywords:

rhythmic coordination;interpersonal coordination;symmetry;drumming;minimal group

Mehnert, AlyssaReconsidering McKinney's Cotton Pickers, 1927–34: Performing Contexts, Radio Broadcasts, and Sound Recordings
PhD, University of Cincinnati, 2018, College-Conservatory of Music: Music (Musicology)
Jazz scholars and musicians consider McKinney’s Cotton Pickers (hereafter MKCP), a Detroit-based dance band active from 1923 to 1941, to be one of the important black dance bands of the 1920s. However, this band has received little attention in jazz scholarship when compared to its contemporaries, the Ellington, Henderson, and Calloway orchestras. John Chilton published a short book on the band, titled McKinney’s Music: A Bio-Discography of McKinney’s Cotton Pickers in 1978, and Gunther Schuller provided detailed analysis of John Nesbitt’s arrangements for the band in his volume The Swing Era: The Development of Jazz, 1930–1945. However, Chilton overlooked many details regarding the band’s history, particularly its radio presence, and Schuller espoused a negative view of many of Donald Redman’s arrangements for the band. This study provides new information regarding MKCP’s performing context, radio broadcasts, recordings for Victor, touring schedule, and audience reception. I draw from newspapers (particularly radio schedules, music criticism, and letters from readers), as well as oral histories, sales catalogues published by Victor records, and transcriptions of MKCP’s recordings. In order to move away from a focus on recordings as autonomous objects in jazz historical writing, this study responds to Lydia Goehr’s call to reconcile the aesthetic and historical by considering how “empirical space” influenced the musical space of MKCP’s recordings. MKCP started as a regional broadcasting dance band and quickly gained status as a nationally recognized dance band and Victor recording artist. After 1930 changes in entertainment industry caused MKCP to shift from its permanent engagement at the Graystone Ballroom and on radio to instead work as a regional touring dance band, like many bands at this time straddling the national and regional. The challenge of these rigorous tours coupled with management problems likely caused the band’s decline. MKCP’s history coincides the developing popularity of radio, the practice of early electrical recording at Victor, and the increase in territory band touring after 1930. Documentation of this band’s popularity as recorded in newspapers and oral histories reveals the nature of audience reception of dance orchestras during this period. Analysis of the MKCP’s recordings shows that the band and its arrangers responded to audience taste for a balance of precision and entertainment. Finally, a revolving door for musicians between the MKCP and the Ellington, Henderson and Calloway Orchestras, coupled with increased touring in the 1930s, contributed to the homogenization of the arranged big band sound that became popular during the Swing Era.

Committee:

bruce mcclung, Ph.D. (Committee Chair); Jenny Doctor, PhD (Committee Member); Daniel Goldmark, Ph.D. (Committee Member); Stephan Meyer, Ph.D. (Committee Member)

Subjects:

Music

Keywords:

jazz;black musicians;McKinneys Cotton Pickers;Don Redman;radio;recording history

Baker, Emily AThe Mediating Role of Dichotomous Thinking in the Formation of Stigmatizing Attitudes Towards Substance Users
MA, University of Cincinnati, 2018, Education, Criminal Justice, and Human Services: Counseling, Mental Health
Substance use is one of the most common mental health concerns, and yet one of the most undertreated. While there are several reasons for this lack of engagement, stigmatizing attitudes is cited as the most common and pervasive barrier to seeking treatment. Understanding the formation of these attitudes is essential to deconstructing them. The current study aims to further understand the underlying formation of these stigmatizing attitudes, specifically the role that individual traits and thinking patterns play. It is hypothesized that dichotomous thinking, or all-or-nothing thinking, will have a mediating effect between the relationship of trait anxiety and stigmatizing attitudes towards substance users. Further, it is predicted that dichotomous thinking will also explain the relationship between intolerance of ambiguity and stigmatizing attitudes towards substance users. We tested the hypotheses using Hayes (2013) PROCESS macro mediation analysis and utilizing data from a larger parent study on perceptions of individuals with substance use disorders. The results indicate that dichotomous thinking has a small significant indirect effect in explaining the relationship in the two mediation models. The results offer an explanation about the impact of individual trait and cognitive differences on stigmatizing attitudes towards substance users, providing more information about attributes that could be targeted to combat societal stigma and its effects. Keywords: stigma, substance use, dichotomous thinking, trait anxiety, intolerance of ambiguity

Committee:

Michael Brubaker, Ph.D. (Committee Chair); Shauna Acquavita, Ph.D. (Committee Member); George Richardson, Ph.D. (Committee Member)

Subjects:

Counseling Psychology

Keywords:

stigma;substance use;dichotomous thinking;trait anxiety;intolerance of ambiguity;substance abuse

Lindquist-Grantz, RobinYouth Participatory Action Research as a Strategy for Adolescent Suicide Prevention
PhD, University of Cincinnati, 2017, Education, Criminal Justice, and Human Services: Educational Studies
After years of decline, there has been a steady increase in the percentage of youth who report seriously considering suicide or making a suicide plan (Kann et al., 2016) and those who have died by suicide (CDC, 2016). As of 2014, suicide moved from being the third leading cause of death for youth ages 10 to 24 to being the second leading cause of death (CDC, 2014). Public strategies and funding to prevent suicide have increased; however, the effectiveness of existing strategies varies, especially in regard to youth help-seeking behaviors, problem-solving, and treatment engagement and utilization. Although youth are among the primary targets of suicide prevention strategies, they have largely been excluded from prevention efforts except as passive participants in programs and research studies. Youth Participatory Action Research (YPAR) is a collaborative approach to research that engages youth and academics as equal partners, and aims to build youth potential for being intentional agents of change for issues that directly affect them and their peers (Cammarota & Fine, 2008). The current study utilized YPAR within an integrated social ecological and positive youth development theoretical framework as an adolescent suicide prevention strategy in Cincinnati, Ohio. Multiple methods were used to determine the usability of YPAR as an innovative approach for building youth development, to explore youth experiences with YPAR for suicide prevention, and to understand youth capacity for conducting suicide prevention research. Findings revealed that confidence gained through YPAR processes contributed to important changes in individual youth development, which then fostered group development and positive perceptions of their capacity to work collectively to address adolescent suicide amongst themselves and with their peer group. The findings have important implications for understanding key processes within YPAR that lead to individual and group development, and where in the YPAR process these changes occur.

Committee:

Lisa Vaughn, Ph.D. (Committee Chair); Jacqueline Grupp-Phelan, M.D. (Committee Member); Farrah Jacquez, Ph.D. (Committee Member)

Subjects:

Public Health

Keywords:

adolescents;youth;suicide prevention;youth participatory action research;participatory research;positive youth development

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