Search ETDs:
THE ROLE OF CDC42 IN THE BUDDING PATTERN OF HAPLOID BUDDING YEAST
Narayan, Monisha

2013, Master of Mathematical Sciences, Ohio State University, Mathematics.
Saccharomyces cerevisiae, commonly known as budding yeast, is a microorganism that has the capability to proliferate rapidly under optimal environmental conditions. Previous studies focused on cells that exhibit symmetry breaking where proteins undergo polarization without spatial cues. In our study, we would like to understand how localization of Cdc42 is established when a spatial cue is present. In wild type haploid cells, the spatial cues interact with a downstream module that includes Rsr1/Bud1 which recruit different proteins including a key protein called Cdc42. A positive feedback loop involving a GDP-GTP exchange factor Cdc24, helps the maintenance of Cdc42 at the bud site. We have created a model to understand how haploid budding yeast wild type cells undergo polarization with spatial cues in order to understand how yeast cells select the next bud site. Simulations have shown that there are two biological possibilities. We conducted corresponding biological experiments in the lab of Dr. Hay-Oak Park. Currently the data shows that two cases are possible. To check the accuracy of our model, 3-D reconstructions will be performed in the future.
Ching-Shan Chou, Ph.D (Advisor)
Hay-Oak Park, Ph.D (Advisor)
Chuan Xue, Ph.D (Committee Member)

Recommended Citations

Hide/Show APA Citation

Narayan, M. (2013). THE ROLE OF CDC42 IN THE BUDDING PATTERN OF HAPLOID BUDDING YEAST. (Electronic Thesis or Dissertation). Retrieved from https://etd.ohiolink.edu/

Hide/Show MLA Citation

Narayan, Monisha. "THE ROLE OF CDC42 IN THE BUDDING PATTERN OF HAPLOID BUDDING YEAST." Electronic Thesis or Dissertation. Ohio State University, 2013. OhioLINK Electronic Theses and Dissertations Center. 26 Sep 2017.

Hide/Show Chicago Citation

Narayan, Monisha "THE ROLE OF CDC42 IN THE BUDDING PATTERN OF HAPLOID BUDDING YEAST." Electronic Thesis or Dissertation. Ohio State University, 2013. https://etd.ohiolink.edu/

Files

NarayanThesis.pdf (1.43 MB) View|Download