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Analysis and Characterization of Microbes from Ancient Glacial Ice
Veerapaneni, Ram S.

2009, Doctor of Philosophy (Ph.D.), Bowling Green State University, Biological Sciences.
The objective of this research was to study the bacterial and fungal composition of icecores from Arctic and the Antarctic regions to understand their geographical and
temporal distribution. Cosmopolitan microbes dominate these environments and have
been deposited primarily by winds, birds, Ocean currents and mammals. Atmospheric
currents play a significant role in the transport of these microorganisms to the polar
regions. We hypothesize that the geographical isolation of the Antarctic region from
other land masses, compared to the Arctic region, affects the transport of the
microorganisms to the Antarctic region, resulting in lower number of microbes entrapped
in the Antarctic ice. Four ice cores each were analysed from the Arctic (GISP2D core)
and the Antarctic (Vostok 5G core) regions dating back to 10,000 YBP, 57,000 YBP,
105,000 YBP and 155,000 YBP using culturing, PCR, sequencing and SEM techniques.
We report the isolation of fungi and bacteria from six of the eight ice cores analysed.
Fungi most closely related to Rhodotorula, Penicillium, Cladosporium, Alternaria,
Aspergillus and Cryptococcus, and bacteria related to Caulobacter and Bacillus were
identified. The ice cores from Arctic had higher number of microorganisms and species
richness than the core sections from the Antarctic region. Phylogenetic studies were done
to compare the organisms with one another and also with the conspecifics. Some
sequences showed high similarity to contemporary species, while some did not group
closely with the present day organisms. Fungi isolated from the different locations, and
closely related to the same genus, did not show high similarity with one another.
Scott Rogers (Advisor)
Kurt Panter (Committee Chair)
Carmen Fioravanti (Committee Member)
Vipaporn Phuntumart (Committee Member)
John Castello (Committee Member)
122 p.

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Veerapaneni, R. (2009). Analysis and Characterization of Microbes from Ancient Glacial Ice. (Electronic Thesis or Dissertation). Retrieved from https://etd.ohiolink.edu/

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Veerapaneni, Ram. "Analysis and Characterization of Microbes from Ancient Glacial Ice." Electronic Thesis or Dissertation. Bowling Green State University, 2009. OhioLINK Electronic Theses and Dissertations Center. 24 Sep 2017.

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Veerapaneni, Ram "Analysis and Characterization of Microbes from Ancient Glacial Ice." Electronic Thesis or Dissertation. Bowling Green State University, 2009. https://etd.ohiolink.edu/

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