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DEVELOPMENT OF NOVEL MULTICHANNEL RESISTIVE PULSE SENSORS FOR MICRO-PARTICLE DETECTION AND DIFFERENTIATION
Jagtiani, Ashish V.

2007, Master of Science, University of Akron, Mechanical Engineering.
In this thesis, high throughput, all electronic resistive pulse sensors for detection and counting of micro-scale particles are developed. The use of multiple sensing channels enables rapid detection of microparticles in comparison to the existing single channel resistive pulse sensors. The detection of particle is based upon Coulter counting principles, that is, when a particle pass through a microchannel, it causes a resistive pulse. The magnitude, duration and polarity of the resistive pulse can be correlated to size, surface charge and concentration of the particles. The multichannel sensors are promising to enable portable devices for rapid onsite detection and differentiation of a variety of bioparticles. The first demonstration of the high throughput multichannel sensor uses four peripheral reservoirs connected to a single central reservoir connected through the fabricated sensing microchannels. This design allows detection of particle in two modes: from the central reservoir towards the four peripheral reservoir allowing improvement in detection rate. The second mode of detection, from the peripheral reservoir towards the central reservoir in addition to improvement in detection efficiency allows detection of different particle samples simultaneously. The second implementation of the multichannel sensor uses soft lithography techniques to fabricate parallel PDMS microchannels with fabricated gold electrodes. This design uses a unique design with sensing microelectrodes placed in the center of the microchannels. This design creates isolation resistances among the channels and thus circumvents the crosstalk problem which is caused by automatic electrical connection among microchannels. Compared to the first implementation, this design has the capability to integrate a large number of microchannels and thus significantly increase the throughput and sampling rate. To demonstrate the feasibility of using such a device, four particle types used in this study include Juniper Scopulorum pollen, Cottonwood pollen, polymethacrylate particles 20 µm and 40 µm in diameter. The two pollen types are roughly 20 µm in diameter. The particles were mixed with deionized water and forced to pass through the microchannels. Voltage pulses resulting from the passage of particles across all the four apertures were recorded and analyzed. In the two device designs presented, results demonstrate that the sensor can detect and count particles through its four sensing channel simultaneously without cross talk. The improvement in the counting efficiency is approximately 300% in comparison to the single channel sensor while maintaining the same accuracy, sensitivity and reliability. The counting efficiency can be further improved by integrating more channels onto the device. Results demonstrate that the device can be used to differentiate between pollen and polymethacrylate particles based on the difference in surface charge. This phenomenon could be applied to differentiate different types of bioparticles.
Jiang Zhe (Advisor)

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Jagtiani, A. (2007). DEVELOPMENT OF NOVEL MULTICHANNEL RESISTIVE PULSE SENSORS FOR MICRO-PARTICLE DETECTION AND DIFFERENTIATION. (Electronic Thesis or Dissertation). Retrieved from https://etd.ohiolink.edu/

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Jagtiani, Ashish. "DEVELOPMENT OF NOVEL MULTICHANNEL RESISTIVE PULSE SENSORS FOR MICRO-PARTICLE DETECTION AND DIFFERENTIATION." Electronic Thesis or Dissertation. University of Akron, 2007. OhioLINK Electronic Theses and Dissertations Center. 22 Aug 2017.

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Jagtiani, Ashish "DEVELOPMENT OF NOVEL MULTICHANNEL RESISTIVE PULSE SENSORS FOR MICRO-PARTICLE DETECTION AND DIFFERENTIATION." Electronic Thesis or Dissertation. University of Akron, 2007. https://etd.ohiolink.edu/

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