Evaluation of Recombinant Salmonella Expressing the Flagellar Protein FliC for Enhanced Immune Responses in Commercial TurkeysAuthor InfoSocial Media
2009, Master of Science, Ohio State University, Animal Sciences.
Salmonella enteritidis is one of the most common causes of human food-borne illness. This organism is often transmitted to humans from poultry products that may have been contaminated pre- or post-harvest. Previous research indicates that oral vaccination of poultry with live attenuated Salmonella can confer a high degree of protection to poultry flocks, thus decreasing the risk of infection in humans. The flagellar protein fliC has shown promise in enhancing an immune response and the ability to protect against challenge of wild-type S. enteritidis in poultry. Several novel attenuated strains of S. enteritidis were developed that express a fliC epitope on the outer membrane protein lamB using the Red recombinase system in combination with overlapping extension PCR. Poults were orally challenged with 106-108 cfu/poult of a wild-type S. enteritidis phage-type 13A (Positive Control), ΔaroA/ΔhtrA SE-M2e (ΔSE M2e), or ΔaroA/ΔhtrA SE-fliC-M2e (ΔSE M2e-fliC) on day-of-hatch and d 21 post-hatch. Liver, spleen, and cecal tonsil were aseptically removed on d 7, 14, 21, 28, 35 and 42 post-hatch to determine colonization and organ invasion. Blood samples were collected on days 7, 14, 21, 28, 35 and 42 post-hatch to determine M2e-specific antibody responses. The ΔSE M2e-fliC candidate vaccine strain demonstrated significantly less organ invasion compared to the ΔSE-M2e strain on d 7 (P <0.0001). However, no marked difference in colonization of the cecal tonsils was noted at d 7. From d 21 through d 42, no significant differences were observed in organ invasion between the vaccine candidate strains when compared to SE13A. The ΔSE M2e-fliC candidate vaccine strain demonstrated a significant decrease in colonization of the cecal tonsils when compared to the ΔSE M2e strain on d 14, 21, and 28 post-immunization (P<0.05). Immunized poults exhibited significantly increased M2e-specific IgG responses, which were further enhanced by the simultaneous expression of fliC on days 14 and 28 (P<0.05). Overall, the ΔSE vaccine candidate strains appear to exhibit reduced organ invasion and colonization while effectively stimulating host immune responses when compared to the wild-type parent strain (SE13A).
Kimberly Cole, PhD (Advisor)
Billy Hargis, PhD/DVM (Committee Member)
Michael Lilburn, PhD (Committee Member)
Salmonella; fliC; vaccine
Kremer, C. (2009). Evaluation of Recombinant Salmonella Expressing the Flagellar Protein FliC for Enhanced Immune Responses in Commercial Turkeys. (Electronic Thesis or Dissertation). Retrieved from https://etd.ohiolink.edu/
Kremer, Courtney. "Evaluation of Recombinant Salmonella Expressing the Flagellar Protein FliC for Enhanced Immune Responses in Commercial Turkeys." Electronic Thesis or Dissertation. Ohio State University, 2009. OhioLINK Electronic Theses and Dissertations Center. 28 Feb 2015.
Kremer, Courtney J. "Evaluation of Recombinant Salmonella Expressing the Flagellar Protein FliC for Enhanced Immune Responses in Commercial Turkeys." Electronic Thesis or Dissertation. Ohio State University, 2009. https://etd.ohiolink.edu/