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Characterization of the Physico-chemical Properties of the Novel Signaling Lipid Diacylglycerol Pyrophosphate
Rapovy, Shannon Marie

2012, BS, Kent State University, College of Arts and Sciences / Department of Biological Sciences.
Diacylglycerol pyrophosphate (DGPP) is an enigmatic phospholipid that has not been fully characterized. DGPP, a phosphorylated form of phosphatidic acid (PA) found in yeast and plants, has a unique chemical feature, namely it carries a pyrophosphate headgroup. PA is a signaling lipid important for many cellular processes including membrane bending/destabilization, stress responses in plants, and protein docking to the membrane. PA-protein interaction can be explained on the basis of the recently formulated electrostatic/hydrogen bond switch model. Turnover of the PA signal in plants can occur via PA’s metabolism to DGPP. Consequently, DGPP is found to increase in concentration following abiotic or biotic stress conditions (drought, salinity, cold, etc.). In order to understand the role of DGPP in biomembranes, we need to know the physicochemical properties of DGPP first. We therefore set out to determine the phase behavior and ionization properties of DGPP in model membrane systems in the presence of lysine rich peptides and calcium. We found that DGPP can act as an electrostatic hydrogen bond donor, its assumed cylindrical shape stabilizes the lamellar phase, and positively charged molecules affect the charge of its pyrophosphate head group. The most significant finding was the strong interaction the phosphomonoester group of DGPP had with lysine residues of polyleucine-alanine synthetic peptides. The possible implications of these results are discussed.
Edgar Kooijman, PhD (Advisor)
Douglas Kline, PhD (Committee Member)
Elizabeth Mann, PhD (Committee Member)
Barbara Yoost (Committee Member)

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Rapovy, S. (2012). Characterization of the Physico-chemical Properties of the Novel Signaling Lipid Diacylglycerol Pyrophosphate. (Electronic Thesis or Dissertation). Retrieved from https://etd.ohiolink.edu/

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Rapovy, Shannon. "Characterization of the Physico-chemical Properties of the Novel Signaling Lipid Diacylglycerol Pyrophosphate." Electronic Thesis or Dissertation. Kent State University, 2012. OhioLINK Electronic Theses and Dissertations Center. 22 May 2015.

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Rapovy, Shannon "Characterization of the Physico-chemical Properties of the Novel Signaling Lipid Diacylglycerol Pyrophosphate." Electronic Thesis or Dissertation. Kent State University, 2012. https://etd.ohiolink.edu/

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