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Role of RNA structures in the initiation of Rous sarcoma virus reverse transcription
Aiyar, Ashok Anantharaman

1994, Doctor of Philosophy, Case Western Reserve University, Biochemistry.
Roles of RNA structures in the 5′ non translated region of Rous Sarcoma Virus (RSV) in the initiation of reverse transcription were evaluated. Besides the previously demonstrated U5-leader stem, reverse transcription of RSV was found to require the U5-IR stem. Disruption of the latter by substitution mutations could be compensated by secondary mutations that restored the structure of the stem but not its sequence. Additionally a role for U5 RNA sequences in base-pairing to the TψC loop sequences in primer tRNA Trp was established. This was examined in vivo and in vitro using a substitution mutation that abrogates the U5-TψC interaction. In vitro this mutation could be rescued by using a primer that contained sequences complementary to the substitution. Further studies revealed that these three regions of duplex RNA structure must be maintained at a specific spatial orientation with respect to each other. Disruption of "spacer" sequences between the duplex regions by insertion and deletion mutations caused defects in initiation. In contrast, substitution mutations in these non base-paired spacer sequences had no effect.
Jonathan Leis (Advisor)
187 p.

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Aiyar, A. (1994). Role of RNA structures in the initiation of Rous sarcoma virus reverse transcription. (Electronic Thesis or Dissertation). Retrieved from https://etd.ohiolink.edu/

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Aiyar, Ashok. "Role of RNA structures in the initiation of Rous sarcoma virus reverse transcription." Electronic Thesis or Dissertation. Case Western Reserve University, 1994. OhioLINK Electronic Theses and Dissertations Center. 03 May 2015.

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Aiyar, Ashok "Role of RNA structures in the initiation of Rous sarcoma virus reverse transcription." Electronic Thesis or Dissertation. Case Western Reserve University, 1994. https://etd.ohiolink.edu/

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