Doctor of Philosophy, The Ohio State University, 2011, Food, Agricultural and Biological Engineering
Pretreatment is a crucial step in the conversion of lignocellulosic biomass to fermentable sugars. Microbial pretreatment with white rot fungi can delignify lignocellulosic biomass under atmospheric conditions and, thus, has the potential to be applied to on-farm wet storage for cost-effective cellulosic ethanol production. The present research investigated the feasibility of solid-state microbial pretreatment of corn stover and other types of lignocellulosic biomass feedstocks with the white rot fungus Ceriporiopsis subvermispora.
The glucose and ethanol yields, based on the theoretical yield of untreated corn stover, reached 66.61% and 57.80%, respectively, after a 35-d pretreatment, which were more than three times of that obtained with the untreated corn stover. Washing of fungal-pretreated corn stover did not cause significant improvement in ethanol yield, suggesting no water insoluble inhibitory compounds were formed during fungal pretreatment. A high correlation was obtained between the remaining lignin content in the treated corn stover and its cellulose digestibility, indicating that lignin removal facilitated enzymatic hydrolysis.
The delignification process of corn stover was investigated throughout a 42-d pretreatment by monitoring changes in composition and enzyme production. Lignin degradation increased with pretreatment time, reaching 39.20% at the end of pretreatment while cellulose degradation was less than 4.52% throughout the process, indicating a high selectivity of the fungus for lignin in the corn stover. However, hemicellulose degradation, mainly xylose loss, was substantial with up to 27.52% hemicellulose simultaneously degraded with lignin. Manganese peroxidase (MnP) and laccase were two detected oxidative enzymes while xylanase was the major hydrolytic enzyme. Observation by scanning electron microscopy showed that fungal pretreatment changed the microstructure of corn stover significantly. At the later stage of pretreatment, corn stov (open full item for complete abstract)
Committee: Yebo Li (Advisor); Ann Christy (Committee Member); Fred Michel (Committee Member); Thaddeus Ezeji (Committee Member)
Subjects: Aeronomy; Agricultural Engineering; Alternative Energy