Two of the most commonly employed bioadhesives used for wound closure applications today are fibrin-based and cyanoacrylate-based bioadhesives, both of which have adverse effects. Fibrin-based bioadhesives allow for the possible transmission of viral blood-borne pathogens, while cyanoacrylate-based bioadhesives have toxicity concerns due to their degradation into formaldehyde. To address these drawbacks and many others, it is proposed that a polyisobutylene-based bioadhesive be employed, since polyisobutylene has a long, successful history as a bio-friendly material. Potential polyisobutylene-based bioadhesives first were prepared by the difunctionalization of α-ω-dihydroxy polyisobutylenes with vinyl methacrylate through “green” enzyme catalyzed Candida antarctica lipase B (CALB) transesterification reactions at 50¿¿¿¿¿¿¿ within 24 hours with high yields. Four different compounded crosslinking solution formulations consisting of synthesized α-ω-dimethacrylate polyisobutylenes, 10% or 20% of the trifunctional crosslinker 2-ethyl-2-hydroxymethyl-1-,3-propanediol trimethacrylate (TMP-TMA) and a 20% solution of the ultra-violet (UV) reactive photoinitiator 2,2-dimethoxy-2-phenylacetophenone effectively demonstrated the ability to crosslink terminally functionalized linear polyisobutylenes into continuous film networks under ambient conditions quickly (< 5 min.) by the use of UV light. Various techniques were used to characterize their crosslinking and physical properties, as well as to determine that the molecular weight of α-ω-dimethacrylate polyisobutylenes had a greater effect on the characterizable attributes than the amount of TMP-TMA employed. Techniques used to characterize the continuous polyisobutylene film networks included: the evaluation of polyisobutylene film discontinuities; the measurement and calculation of their physical dimensions; aesthetic evaluation; solvent extraction and swelling assessments; FTIR; TGA; and DSC. These methods characterized th (open full item for complete abstract)