Master of Science, The Ohio State University, 2022, Food, Agricultural and Biological Engineering
The use of plant-based proteins to replace animal proteins is gaining interest from consumers and
food processors, leading to forecasts that plant proteins to become a major ingredient commodity.
Even though the major commercial plant proteins used today (e.g., soybean and pea) are good
texturizing proteins as evidenced by their widespread use in plant-based analogues, they do not
show good cohesive and viscoelastic properties, which are provided by incorporation of some
seriously criticized ingredients (e.g., gluten, methylcellulose). In this study, we described some
natural and food-friendly ways to promote viscoelasticity and cohesiveness of the corn protein,
zein, to be used in plant-based analogues instead of wheat gluten or hydrocolloids to confer the
desired viscoelastic properties. Rheological and textural measurements showed that arginine and
calcium hydroxide addition to zein-pea protein blends significantly increased elasticity and
cohesiveness. Secondary structure composition analysis through FT-IR emphasized the
importance of -sheet content in enhancing the material viscoelastic properties, specifically
arginine and calcium hydroxide-incorporated blends exhibited the highest -sheet content.
Imaging on blends through scanning electron and confocal microscopy displayed that zein formed
viscoelastic fibrils holding the structure together through a dispersed network. Overall, zein, along
other natural and food approved ingredients, can be the primary agent providing cohesiveness and
viscoelasticity to plant-based formulations and the basis to generate green-label products
formulations and products.
Furthermore, the zein-pea protein-based formulation allows for the adjustment of the textural
properties by varying the calcium hydroxide concentration in the formulation to match the textural
properties of commercial meat and cheese products. Concerning the formulations viscoelastic
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properties, weak frequency dep (open full item for complete abstract)
Committee: Osvaldo Campanella (Advisor); Rafael Jimenez-Flores (Committee Member)
Subjects: Food Science