Doctor of Philosophy, The Ohio State University, 2021, Nursing
Background. African American women experience preterm birth (PTB)—births <37 completed weeks gestation—at 1.5 times the rate of non-Hispanic white women. This persistent health inequity leads to high infant mortality and high morbidity rates for surviving infants. Neighborhood disorder (e.g., housing vacancy, litter, crime) has been related to maternal psychological distress and PTB. Higher levels of neighborhood disorder, maternal psychological distress, and increased risk of PTB have been related to epigenetic modifications, and specifically to DNA methylation (DNAm) of stress-related, glucocorticoid (GC) pathway genes. To our knowledge, no published study has examined the relationship between perceived neighborhood disorder, maternal psychological distress, DNAm of GC pathway genes, and PTB in general or within the highest risk group among U.S. women–African Americans. The purpose of this study was to examine the relationships among perceived neighborhood disorder, maternal psychological distress, DNA methylation of six candidate GC pathway genes (AVP, CRH, CRHBP, FKBP5, HSD11B2, NR3C1), and gestational age at birth in pregnant African American women.
Methods. This prospective cohort study was a subsample of women who participated in the NIH R01 study, Social stressors and inflammation: A mixed methods approach to preterm birth (R01MD001575, PI Giurgescu). Forty-four African American women—22 women with preterm birth and 22 women with full-term birth (FTB)—18-45 years of age and 8-18 weeks gestation were selected for the study. The women completed questionnaires and had peripheral blood samples collected for DNAm analysis.
Results. We report 14 differentially methylated (FDR < 0.05) cytosine-phosphorus-guanine (CpG) dinucleotides between women with PTB and women with FTB. In addition, perceived neighborhood disorder was significantly associated (p<0.05) with average methylation beta levels at 15 dinucleotides in five of six candidate genes, perceived neigh (open full item for complete abstract)
Committee: Cindy Anderson (Advisor); Carmen Giurgescu (Committee Member); Jodi Ford (Committee Member); Alai Tan (Committee Member); Amy Mackos (Committee Member)
Subjects: Genetics; Health Care; Nursing; Obstetrics