Doctor of Philosophy, The Ohio State University, 2020, Horticulture and Crop Science

An intensive production system is used to produce greenhouse floriculture crops, marketed for their flowers and attractive foliage. Chemical, environmental, and cultural methods are used to manage biotic and abiotic stresses during production. Additional tools are needed by growers because of growing concerns around the negative impact of plant production on humans and the environment. The objective of this research was to evaluate potential tools to improve floriculture crop resilience under stress during production and post-production. Botrytis cinerea causes disease in most major greenhouse crops and is resistant to several fungicides. Additional control methods, like plant growth promoting bacteria (PGPB) that can improve plant performance by increasing plant resilience to stress are needed. A collection of 60 bacterial strains was evaluated in a dual culture assay and an initial greenhouse trial with Petunia × hybrida `Carpet Red Bright' to identify strains for the biocontrol of B. cinerea. Daily flower disease severity ratings were used to select seven strains that were evaluated in the validation greenhouse trial. Three Pseudomonas strains were selected for the greatest reduction in B. cinerea infection. The efficacy of PGPB and the plant's susceptibility to B. cinerea were affected by fertilization. Petunia × hybrida `Carpet Red Bright' was treated with bacteria or a commercial biocontrol product and fertilized with synthetic chemical or organic fertilizer at a low or high rate. Measured plant growth and flower disease severity revealed that plants with the high rate synthetic fertilizer were the largest and had the lowest disease severity. Reduction of disease severity varied between bacterial and fertilizer treatment combinations. Plants treated with one bacterium had reduced disease severity at the high rate synthetic chemical fertilizer but not at the low rate organic fertilizer. Specific fertility programs provide crops with needed macro and mi (open full item for complete abstract)

Committee: Michelle Jones (Advisor); David Francis (Committee Member); Francesca Hand (Committee Member); Pablo Jourdan (Committee Member); Matthew Kleinhenz (Committee Member) Subjects: Agriculture; Horticulture