Doctor of Philosophy, The Ohio State University, 2023, Plant Pathology

Plants exist in a constantly evolving microbial environment that significantly influences their growth, development, and overall well-being. Within this microbial milieu, certain bacteria play a pivotal role in enhancing plant health and growth. These beneficial bacteria are collectively referred to as plant growth-promoting bacteria (PGPB). They offer valuable services to plants, including improved nutrient absorption, heightened growth stimulation, and increased resilience against pathogens and the other environmental adversities. PGPB engage with plants through diverse modes of interaction, such as root colonization, endophytic association, or rhizosphere competence. An in-depth comprehension of the molecular mechanisms and ecological dynamics governing these interactions is essential for unlocking the potential of PGPB in promoting sustainable agriculture and environmental remediation. In Chapter 1, I provide an overview of current methods used to detect and diagnose Pseudomonas syringae. This encompasses traditional approaches like culture isolation and microscopy, as well as modern techniques such as PCR and ELISA. Furthermore, I explore the upcoming advancements in this domain, emphasizing the necessity for highly sensitive and specific methods to detect pathogens even at low concentrations. Additionally, I delve into approaches for diagnosing P. syringae infections when they coexist with other pathogens. Chapter 1 Figures can be found in Appendix A. In Chapter 2, I present a significant protocol for monitoring the progression of gray mold fungal infection at various developmental stages of strawberries. I detail three distinct in vivo inoculation methods for Botrytis cinerea on strawberry plants, focusing on early, middle, and late stages of strawberry growth. Chapter 2 Figures can be found in Appendix B. In Chapter 3, I introduce Bacillus proteolyticus OSUB18 as a novel inducer of ISR (Induced Systemic Resistance). This bacterium enhances plants' r (open full item for complete abstract)

Committee: Ye Xia (Advisor); Christopher Taylor (Committee Member); Yu (Gary) Gao (Committee Member); Lisa (Beck) Burris (Committee Member); Jonathan Jacobs (Committee Member) Subjects: Agriculture; Agronomy; Biochemistry; Bioinformatics; Biology; Botany; Cellular Biology; Plant Biology; Plant Pathology; Plant Sciences