Doctor of Philosophy, The Ohio State University, 2009, Environmental Science
Human activities involved with urban development have substantial impact on soil. This work examined anthropogenic influences measured by changes in soil profile, length of urbanization period, and the distance from paved roads on soil ecosystems, specifically on the size of key soil nutrient pools, and the abundance and diversity of soilborne nitrogen fixing bacteria and soil nematodes. Further spatial organization of the urban soil nematode community was described at functional guild and taxonomic level to explore ways to improve the value of nematodes as bioindicators. First, experimental plots with four different soil matrixes which are commonly found in newly developed urban sites (topsoil, compost amended topsoil, subsoil and compost amended subsoil) were established. The abundance and diversity of soilborne nitrogen fixing bacteria using gene marker, nifH were monitored for one-year period after tall fescue lawn establishment. Initially, abundance of nifH measured by quantitative polymerase chain reaction was significantly lower in subsoil compared to topsoil, but it enriched quickly to the level in topsoil over a one year period. However, the composition of nifH differed between topsoil and subsoil plots. Second, the sizes of key soil nutrient pools were measured and compared along temporal and spatial gradients related to urban development. Study sites were selected from same land use type; turfgrass-covered soils in public schools. Total soil carbon, nitrogen, organic matter, pH, and exchangeable Ca varied in a predictable manner with duration of urbanization and distance from paved roads. To evaluate the potential of soil nematodes as indicators for detecting differences in urban soil chemistry, soil nematodes were extracted, counted, and identified to the genus level. Along the urbanization duration gradient, soil nematode abundance and diversity indices clearly reflected differences in soil chemistry. The abundance of soil nematodes in road-side soils w (open full item for complete abstract)
Committee: Parwinder Grewal PhD (Advisor); John Cardina PhD (Committee Member); Richard Dick PhD (Committee Member); Edward McCoy PhD (Committee Member); Brian McSpadden Gardener PhD (Committee Member)
Subjects: Ecology