Master of Science, The Ohio State University, 2015, Plant Pathology
Corn fields in Ohio were surveyed in 2013 and 2014 to determine the frequency and abundance of plant-parasitic nematodes. Soil samples were collected at a depth of 40 to 50 cm from 425 fields when corn was between growth stages V3 and V6. A total of 15-16 fields were sampled in each of 28 counties, across 6 soil regions, representing three cropping systems and three tillage programs. Standard laboratory techniques were used for processing and extraction, and plant-parasitic nematodes were identified to genus. Nine commonly occurring morphological groups of plant-parasitic nematodes, namely spiral (Helicotylenchus spp.), lesion (Pratylenchus spp.), lance (Hoplolaimus spp.), dagger (Xiphinema spp.), stunt (Tylenchorhynchus spp.), pin (Paratylenchus spp.), ring (Criconemella spp.), stubby-root (Paratrichodorus spp.), and cyst (Heterodera spp.), along with several genera in the subfamily Tylenchinae counted together as “tylenchids”, were identified, with maximum individual-field population densities ranging from 26 to 1,164 nematodes/100 cm3 soil, depending on the group. Generalized linear mixed models were fitted to the data to estimate county-level heterogeneity in nematode presence, while binary and ordinal logistic regression models were fitted to estimate the odds of each genus being present, and the lesion, lance, spiral, and pin nematodes at potentially damaging population densities based on soil region, cropping sequence, tillage, and soil pH, silt content, and electrical conductivity. The spiral nematodes and tylenchids were detected in 94 and 96% of the fields, whereas the lesion, pin, lance, stunt, and dagger nematodes were recovered from 80, 57, 48, 48, and 37% of the fields, respectively. The stubby-root, cyst, and ring nematodes were the least frequent, each identified from fewer than 13% of the fields. County-level heterogeneity varied among the genera, with variance estimates ranging from 0.56 to 3.23. However, except for comparisons between the stunt an (open full item for complete abstract)
Committee: Pierce Paul Dr. (Advisor); Terry Niblack Dr. (Committee Member); Laura Lindsey Dr. (Committee Member)
Subjects: Plant Pathology