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  • 1. Massie, Ernest Thyroid hormone effects on testicular metabolism and function /

    Master of Science, The Ohio State University, 1968, Graduate School

    Committee: Not Provided (Other) Subjects:

  • 2. Toennies, Marc Effect of cryptorchidism on testicular 5 alpha-reduced androgen formation and hypothalamo-hypophyseal secretions in the adult male rat.

    Master of Science, The Ohio State University, 1978, Graduate School

    Committee: Not Provided (Other) Subjects:

  • 3. Free, Michael Gas tensions in spermatic and peripheral blood of normal and heat treated rams /

    Master of Science, The Ohio State University, 1965, Graduate School

    Committee: Not Provided (Other) Subjects:

  • 4. Golconda, Sarah Why is Nature Able to Mold Some Phenotypes More Readily than Others? Investigating the Structure, Function and Evolution of ßeta-2 Tubulin in Drosophila Melanogaster

    Master of Science (M.S.), University of Dayton, 2018, Biology

    Some phenotypes have more ability to evolve than others, captured by the term “evolvability.” While some traits can evolve rapidly, such as the shape, color and size of a butterfly wing, the Drosophila testis specific beta-2 (ß2) tubulin protein, a fundamental component of the spermtail axonemes, has not evolved in over 60 million years. This protein is a main element of the microtubules within the axoneme which supports the motility of the sperm cell. There is a 9+2 configuration of microtubules, nine doublets of microtubules arranged along the outer edge of the structure with two central microtubules. Each microtubule consists of tubulin dimers of ß2 tubulin and the major alpha tubulin isoform 84B which is present in most cells of the body. Previous studies have shown that substitutions of the of beta-1 tubulin, a 95% identical paralog of beta-2 expressed in somatic cells, and chimeric tubulins composed of beta-1 and beta-2 tubulin sequence are unable to support a motile axoneme, indicating the axoneme is highly sensitive to beta tubulin structure. From these findings, evolutionary conservation and highly sensitive structure/ function relationship, two hypotheses tested here were developed for the long conservation of ß2 tubulin. The first, stabilizing selection: nature is constantly selecting a particular sequence even though other sequences may work due to differences in the quality of sperm produced. Or, it may be that there is no alternative sequences that function, and a co-evolutionary event with another protein found within the axoneme is required to release beta-2 tubulin to evolve. These hypotheses were tested using the substitution of a beta-2 ortholog, the gene in a different species which evolved from a common ancestor, was examined to determine its ability to produce a functional sperm in the Drosophila melanogaster model. If able to produce a functional sperm, stabilizing selection is supported; if unable, a co-evolutionary event has occurred. Throug (open full item for complete abstract)

    Committee: Mark Nielsen (Committee Chair) Subjects: Biology; Developmental Biology; Evolution and Development

  • 5. Lynn, Rita Phosphofructokinase in the rat : influence of temperature and sexual maturation on the testicular form : testosterone dependence of the seminal vesicle form /

    Doctor of Philosophy, The Ohio State University, 1976, Graduate School

    Committee: Not Provided (Other) Subjects: Biology

  • 6. Nguyen, Duc Testicular blood flow and testosterone secretion rates in the rabbit and rat /

    Doctor of Philosophy, The Ohio State University, 1975, Graduate School

    Committee: Not Provided (Other) Subjects: Biology

  • 7. Hall, Robert The effects of pharmacologically-induced reversible aspermatogenesis and cryptorchidism on serum estrogens of the male rat /

    Doctor of Philosophy, The Ohio State University, 1974, Graduate School

    Committee: Not Provided (Other) Subjects: Biology

  • 8. Wisner, James Androgen biosynthetic capabilities of isolated seminiferous tubules, interstitial and whole testis tissues of normal and cryptorchid rats /

    Doctor of Philosophy, The Ohio State University, 1973, Graduate School

    Committee: Not Provided (Other) Subjects: Biology

  • 9. Schanbacher, Bruce Testicular carnitine acetyltransferase activity and serum testosterone levels in developmental stages of the rat and ram /

    Doctor of Philosophy, The Ohio State University, 1973, Graduate School

    Committee: Not Provided (Other) Subjects: Biology

  • 10. Cheng, Hsien Isoenzymes of hexokinase in rat testes at various developmental and endocrine states /

    Doctor of Philosophy, The Ohio State University, 1971, Graduate School

    Committee: Not Provided (Other) Subjects: Biology

  • 11. Vera Cruz, Nestor The effects of androgenic, estrogenic and gonadotrophic hormones in vitro on the metabolic activity of normal and cryptorchid rat testicular tissue /

    Doctor of Philosophy, The Ohio State University, 1968, Graduate School

    Committee: Not Provided (Other) Subjects: Agriculture

  • 12. Free, Michael Radiorespirometric studies on glucose metabolism in mammalian and avian testes /

    Doctor of Philosophy, The Ohio State University, 1967, Graduate School

    Committee: Not Provided (Other) Subjects: Biology

  • 13. Dasgupta, Shuvalaxmi PP1 Gamma 2 LEVELS ARE HIGHLY REGULATED IN TESTIS TO ENSURE NORMAL SPERMATOGENESIS AND MALE FERTILITY

    BS, Kent State University, 2013, College of Arts and Sciences / Department of Biological Sciences

    The mammalian male reproductive system is comprised of the testis, encased in connective tissue Tunica albuginea and an intricate system of interconnected ducts. Both exocrine and endocrine in nature, the primary functions of the testis are to synthesize and secrete male sex hormones and generate sperms by the process of spermatogenesis. Sperm develops in the environment of the seminiferous tubules, which converges on the rete testis and leads out to a collective duct system called Epididymis [fig 1]. The Sertolli or Sustentacular cells lining the Epididymis sustain the proper environment required for spermatozoa development. One of the major roles of the Sertolli cells is to define two physiologically distinct compartments in the seminiferous tubule. Spermatogenesis is a continuous process by which primary male germ cells in the seminiferous tubules produce mature male gametes, the spermatozoa. It begins with diploid spermatogonial cells dividing by the process of mitosis to produce the primary spermatocytes. The primary spermatocytes generated by the process of spermatocytogenesis move into the tubule, where it’s DNA is duplicated. The primary spermatocytes then undergo meiosis I to produce haploid secondary spermatocytes. These haploid spermatocytes rapidly enter meiosis II to produce spermatids by the process of spermatidogenesis. Each spermatid condenses the DNA contained within it, develops a tail and an acrosome giving rise to an immotile spermatozoa [fig 2]. These are then transported to the epididymis where they become motile [1]. Phospho protein phosphatases, abbreviated as PPP, are enzymes that dephosphorylates certain phosphorylated proteins. Proteins are phosphorylated on nine amino acids, for example serine, threonine, tyrosine, cystine, etc. Of these, serine, threonine and tyrosine phosphorylation is predominant in eukaryotic cells and plays a role in key regulatory mechanisms. PPP1 belongs to protein serine/threonine phosphatases. Protein (open full item for complete abstract)

    Committee: S Vijayaraghavan Dr (Advisor); D Kline Dr (Committee Member); S Newman Dr (Committee Member); S Roxburgh Dr (Committee Member) Subjects: Biochemistry; Biology; Biomedical Engineering; Biomedical Research

  • 14. Ramdas, Shandilya Role of PP1γ2 Binding Partners in Spermatogenesis and Sperm Function

    PHD, Kent State University, 2012, College of Arts and Sciences / Department of Biological Sciences

    PP1γ2 (PPP1CC2) is a serine/threonine phosphatase that is highly expressed in testis and the only isoform expressed in mature spermatozoa. It belongs to a group of highly conserved serine/threonine phosphatases known as PP1 and is formed by the alternate splicing of the Ppp1cc gene. PP1 function is regulated by a large number of proteins that either regulate its activity or bind to it and target it to different regions of a cell. Male mice lacking Ppp1cc gene are infertile whereas female mice are normal. This is due to a defect in spermiogenesis. My first aim involved in analyzing the role of RSPH1, a protein highly expressed in the radial spoke of mature spermatozoa. A previous report showed that RSPH1 is expressed in two isoforms - a larger testicular isoform – P44 and a smaller epididymal isoform – P30. We show that P30 is expressed only in the caput and caudal regions of mouse epididymis and not in the caput. This suggested that P30 could be playing a role in the epididymal maturation of sperm and warranted a closer look at the role of P30 in sperm function. During the course of our studies, we showed by microsequencing that P30 is not an isoform of RSPH1 and is in fact another epididymal protein CRISP1. Considering the phenotypic similarity between Ppp1cc and Rsph1 knockout mice, we wanted to analyze the amounts of PP1γ2 and its binding partners I2, I3 and sds22 in Rsph1 knockout mouse testis and epididymis. We observed no difference in levels of these proteins when compared to wild-type mice. My second aim involved the identification and characterization of testis-specific isoforms of ubiquitously expressed ancient binding partners of PP1 – I2, I3 and sds22. Our laboratory had previously observed I2-like activity in bovine sperm but had never been able to show the physical presence of I2 in sperm. Using specific antibodies against I2, we were able to, for the first time, conclusively provide evidence for the presence of I2 in testis and sperm. We showed the pr (open full item for complete abstract)

    Committee: Srinivasan Vijayaraghavan (Advisor); Douglas Kline (Committee Member); Yijing Chen (Committee Member); Wen-Hai Chou (Committee Member); Soumitra Basu (Committee Member) Subjects: Biology