Doctor of Philosophy, The Ohio State University, 2024, Plant Pathology
Often overshadowed by staple crops, vegetables contain many essential vitamins and minerals and play a key role in global food and nutritional security. However, vegetable production is threatened by a variety of diseases, including bacterial wilt and bacterial spot. My dissertation research utilizes genomic surveys to elucidate the diversity of the bacteria causing these diseases, both globally and locally. Bacterial wilt disease is endemic within the country of Cambodia, causing significant yield losses for Cambodian growers. However, the diversity of the Ralstonia solanacearum Species Complex (RSSC), the causal agent responsible for bacterial wilt disease, has not been defined in Cambodia. Therefore, we conducted a bacterial wilt survey within Cambodia, collecting RSSC isolates from four distinct host plants (tomato, hot pepper, long bean, and bitter gourd) over three locations, for a total of 24 RSSC isolates. We found that all 24 of the Cambodian RSSC isolates belong to phylotype I and are classified as Ralstonia pseudosolanacearum. Through disease progress assays on susceptible hosts, we observed that variation in the Cambodian isolate's ability to cause consistent wilt was dependent on the method of inoculation. Additionally, the Cambodian R. pseudosolanacearum isolates exhibited a wide range of phylogenomic diversity. When comparing the core and accessory genome and the Type III effector profile of the Cambodian isolates, we found that the R. pseudosolanacearum accessory genome better reflected the host of isolation and host range of the isolates compared to the core genome. Altogether, this research provides a glimpse into the RSSC diversity present within Cambodia and insight into R. pseudosolanacearum host range. Bacterial spot disease affects tomato and pepper production worldwide and is caused by a species complex of Xanthomonas bacteria: X. hortorum pv. gardneri, X. euvesicatoria pv. euvesicatoria, X. euvesicatoria pv. perforans, and X. vesicatoria. We (open full item for complete abstract)
Committee: Jonathan Jacobs (Advisor); Mary Rodriguez (Committee Member); Francesca Hand (Committee Member); Sally Miller (Advisor)
Subjects: Plant Pathology