Doctor of Philosophy, The Ohio State University, 2005, Horticulture and Crop Science
The objective of this study was to increase the resistance of crops (tobacco and tomato) to water deficit stress. To achieve this goal transgenic plants were generated using two genes, a putative tomato type I inositol 5 polyphosphatase (5PTase) that terminates inositol 1,4,5 trisphosphate (IP3) signaling and ABRE binding factor ABF4 derived from Arabidopsis. Inositol 1,4,5 triphosphate (IP3), as one of phosphoinositides, is known to transduce a stress signal by changing its level in response to water deficit, osmotic stress, and low temperature in plants. Recently it was shown that type I inositol 5 phosphatases (5PTases), At5PTase1 and AtIP5PII/At5PTase2, regulate IP3 level in plant like in animals and that up-regulation of these genes decreases IP3 levels which leads to a reduction in the expression of ABA-/drought-responsive genes in Arabidopsis. On the basis of sequence similarity to Arabidopsis 5PTases, four tomato cDNAs (Le5PT1-4) that encode putative tomato type I 5PTase proteins were identified. Predicted protein sequences of identified Le5PTs had conserved catalytic domains that are required for 5PTase enzyme activity. Two clones, Le5PT1 and Le5PT2 were similar to AtIP5PII/At5PTase2 and At5PTase1, respectively. The expression of Le5PT1 was down-regulated in early time point under dehydration, NaCl, and exogenous ABA treatment, indicating that Le5PT1 may play a negative role in stress signaling. Transgenic tobacco plants with 35S:Le5PT1 did exhibit weak expression of the drought inducible gene, NtERD10B, but did not show correlation with resistance to water deficit stress. AtABF4, a bzip transcription factor, is known to induce the expression of ABA-responsive genes. The expression of Arabidopsis ABF4/AREB2 gene under the control of guard cell specific KST1 promoter was shown to significantly increase drought tolerance in tobacco and tomato plants. The transgenic plants exhibit significantly lower water loss per unit leaf area compared to wild type plants. (open full item for complete abstract)
Committee: James Metzger (Advisor)
Subjects: Biology, Plant Physiology