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Maosah FINAL 4 30 2021 with cert.pdf (1.75 MB)

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Enhancing transglycosylation reaction by minimizing hydrolysis in oligosaccharide synthesis

Maosah, Charity Kwamboka
http://rave.ohiolink.edu/etdc/view?acc_num=ysu161990153240475

Abstract Details

2021, Master of Science in Chemistry, Youngstown State University, Department of Biological Sciences and Chemistry.
Oligosaccharides are a class of carbohydrates which play important roles in several areas including therepeutics, food, phamaceuticals, animal feed and their use as prebiotics. Current commercial production of oligosaccharides involves lengthy multistep procedures with lowbyields. To improve oligosaccharide yield, a broad range of factors related to oligosaccharide synthesis by transglycosylation was evaluated. Three enzymes, β-galactosidase from Aspergillus oryzae, lactase from lactose intolerance supplements, and Enterobacter β-galactosidase were tested for their ability to catalyze a transglycosylation reaction. The enzymes were applied in both free and immobilized forms and the activities toward transglycosylation were compared. Immobilization of enzymes led to increased oligosaccharide synthesis and improved enzyme stability under different conditions. Lactose, which is a main milk carbohydrate, was used as the major substrate for synthesis of oligosaccharides. Factors like organic solvents, temperature, and pH were varied with the aim of obtaining the conditions with higher transglycosylation results. The analysis of products was done by thin layer chromatography. The yield of transglycosylation products was dependent on both temperature and pH. The preferred conditions for formation of oligosaccharides are pH 8 and 50 - 60 °C. High concentrations of glucose and galactose, the products of lactose hydrolysis, suppressed both transglycosylation and hydrolysis reactions. When sucrose and lactose were used together, an increase in the sucrose to lactose ratio led to an increase in transglycosylation products. Addition of alcohols resulted in formation of alkyl-glycosides instead of oligosaccharides. Enterobacter β-galactosidase was successfully overexpressed in E. coli cells and demonstrated transglycosylation and hydrolysis activity.
Nina Stourman, PhD (Advisor)
Michael Serra, PhD (Committee Member)
Jonathan Caguiat, PhD (Committee Member)
87 p.
Biochemistry; Chemistry
Transglycosylation; Hydrolysis; Glycosidases; Oligosaccharide

Recommended Citations

Citations

  • Maosah, C. K. (2021). Enhancing transglycosylation reaction by minimizing hydrolysis in oligosaccharide synthesis [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu161990153240475

    APA Style (7th edition)

  • Maosah, Charity. Enhancing transglycosylation reaction by minimizing hydrolysis in oligosaccharide synthesis . 2021. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu161990153240475.

    MLA Style (8th edition)

  • Maosah, Charity. "Enhancing transglycosylation reaction by minimizing hydrolysis in oligosaccharide synthesis ." Master's thesis, Youngstown State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=ysu161990153240475

    Chicago Manual of Style (17th edition)

ysu161990153240475
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© 2021, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.