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Changes in Gene Expression of Neurospora crassa in Response to Quinic Acid

Brown, Kayla A.
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1484090465840537

Abstract Details

2016, Master of Science in Biological Sciences, Youngstown State University, Department of Biological Sciences and Chemistry.
As a filamentous fungus, Neurospora crassa serves as an ideal model for eukaryotic organisms. Like many fungi, Neurospora is able to utilize many different carbon sources for energy. This however, requires the presence of genes that code for a variety of metabolic pathways that are not always needed. An example of such a group of genes would be the genes involved in utilizing quinic acid. When Neurospora grows in the presence of a less preferred carbon source, such as quinic acid, gene expression of the quinic acid (qa) gene cluster is up-regulated. This allows the organism to metabolize quinic acid and survive in the less favorable conditions. In contrast, when in the presence of a preferred carbon source, such as dextrose or sucrose, the qa genes are repressed. This study examines how changing the carbon source effects gene expression in wild-type N.crassa. N.crassa was first grown in presence of either quinic acid or sucrose and harvested for tissue. Then, protein was extracted from this tissue and analyzed by 1- Dimensional Gel Electrophoresis (1-DGE). Differences in protein expression was compared using the Quantity One® 1-D analysis software. Proteins unique to growth on quinic acid were identified after being submitted for mass spectrometry. Finally, gene transcription was quantitated in order to determine which genes coded for proteins expressed only in the presence of quinic acid by reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).
David K. Asch, PhD (Advisor)
Gary R. Walker, PhD (Committee Member)
Jonathan J. Caguiat, PhD (Committee Member)
50 p.
Bioinformatics; Biology; Cellular Biology; Molecular Biology
Neurospora crassa, quinic acid, RT-qPCR, protein profiles

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Citations

  • Brown, K. A. (2016). Changes in Gene Expression of Neurospora crassa in Response to Quinic Acid [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1484090465840537

    APA Style (7th edition)

  • Brown, Kayla. Changes in Gene Expression of Neurospora crassa in Response to Quinic Acid. 2016. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1484090465840537.

    MLA Style (8th edition)

  • Brown, Kayla. "Changes in Gene Expression of Neurospora crassa in Response to Quinic Acid." Master's thesis, Youngstown State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1484090465840537

    Chicago Manual of Style (17th edition)

ysu1484090465840537
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© 2016, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.