Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


ysu1251756763.pdf (1.63 MB)

ETD Abstract Container

Abstract Header

Creation of a Site-Directed Mutant of Hen Egg White Lysozyme Working Toward Site-Specific Oxidation as it Relates to Protein Structure

Mensah, Eric
http://rave.ohiolink.edu/etdc/view?acc_num=ysu1251756763

Abstract Details

2009, Master of Science in Chemistry, Youngstown State University, Department of Chemistry.
Metal catalyzed oxidation of protein involves a reaction between hydrogen peroxide and protein-bound metal ions. Production of the highly-reactive hydroxyl radical leads to oxidative damage in the immediate vicinity of the bound-metal ion. To examine the relationship between protein structure and oxidative damage a series of site-directed mutants of hen egg white lysozyme (HEWL) were created. This project focuses on generation of the site directed mutant where residue His15 will be altered to a serinyl residue (H15S), also work on the preliminary purification and characterization of the putative mutant would be touched on.
Michael Serra, PhD (Advisor)
John Jackson, PhD (Committee Member)
Peter Norris, PhD (Committee Member)
64 p.
Biochemistry; Organic Chemistry
protein expression; site-specific oxidation; site-directed mutagenesis; protein purification; protein modification; reactive oxygen species and disease state

Recommended Citations

Citations

  • Mensah, E. (2009). Creation of a Site-Directed Mutant of Hen Egg White Lysozyme Working Toward Site-Specific Oxidation as it Relates to Protein Structure [Master's thesis, Youngstown State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1251756763

    APA Style (7th edition)

  • Mensah, Eric. Creation of a Site-Directed Mutant of Hen Egg White Lysozyme Working Toward Site-Specific Oxidation as it Relates to Protein Structure. 2009. Youngstown State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ysu1251756763.

    MLA Style (8th edition)

  • Mensah, Eric. "Creation of a Site-Directed Mutant of Hen Egg White Lysozyme Working Toward Site-Specific Oxidation as it Relates to Protein Structure." Master's thesis, Youngstown State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1251756763

    Chicago Manual of Style (17th edition)

ysu1251756763
1,428
© 2009, all rights reserved.
This open access ETD is published by Youngstown State University and OhioLINK.