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Carbaryl Exposure to Danio rerio Leads to Activation of the Aryl Hydrocarbon Receptor Pathway

Barnhisel, Taylor
http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617979942441695

Abstract Details

2021, Bachelor of Science, Wittenberg University, Biology.
Insecticide use is steadily increasing in the United States. Of these, the insecticide Sevin™ has the active ingredient carbaryl, which is a xenobiotic. This is a reverse inhibitor of acetylcholinesterase, which prevents nerve excitation causing paralysis and death in insects. Though insects are the target species, carbaryl has many routes of exposure such as run-off, inhalation, and physical contact making it easy to encounter for non-target species like aquatic life and humans. Carbaryl has been studied within in vitro models to test its effects on zebrafish. The insecticide has already been shown to act on the acetylcholinesterase (AChE) pathway, but it also has potential to act on a second pathway: the aryl hydrocarbon receptor (AhR) pathway. Zebrafish genes and genetic pathways are homologous to humans, allowing for insight on how carbaryl could be affecting humans as well. This study set out to use an in vivo model to show how carbaryl is inducing cytochrome P450 or other AhR pathway gene expression. Gene expression of carbaryl treated embryos were compared to untreated embryos to examine this possible relationship. Cyp1a and cyp1b gene expression was increased with an increase in carbaryl concentration suggesting that carbaryl may be causing induction of the AhR pathway.
Michelle McWhorter (Advisor)
Matthew Collier (Committee Member)
Daniel Marous (Committee Member)
20 p.
Agricultural Chemicals; Biochemistry; Biology; Developmental Biology; Environmental Science; Genetics; Molecular Biology; Toxicology
insecticides; Sevin; carbaryl; xenobiotic; gene expression; AhR pathway; aquatic zebrafish; Danio rerio; pesticides; zebrafish embryos; cytochrome P450 genes; pesticide pollution; carbamates; pesticide toxicology; teratogens

Recommended Citations

Citations

  • Barnhisel, T. (2021). Carbaryl Exposure to Danio rerio Leads to Activation of the Aryl Hydrocarbon Receptor Pathway [Undergraduate thesis, Wittenberg University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617979942441695

    APA Style (7th edition)

  • Barnhisel, Taylor. Carbaryl Exposure to Danio rerio Leads to Activation of the Aryl Hydrocarbon Receptor Pathway. 2021. Wittenberg University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617979942441695.

    MLA Style (8th edition)

  • Barnhisel, Taylor. "Carbaryl Exposure to Danio rerio Leads to Activation of the Aryl Hydrocarbon Receptor Pathway." Undergraduate thesis, Wittenberg University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617979942441695

    Chicago Manual of Style (17th edition)

wuhonors1617979942441695
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© 2021, all rights reserved.
This open access ETD is published by Wittenberg University Honors Theses and OhioLINK.