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Development and investigation of antibiotic resistance in E. coli using aminoglycosides

Malott, Bradley
http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617617698561944

Abstract Details

2019, Bachelor of Arts, Wittenberg University, Chemistry.
The use of antibiotics to cure bacterial infections is one of the single most significant medical advancements in human history. This research focuses on the ability of bacteria to develop resistance to these antibiotics. The aminoglycoside antibiotic kanamycin was used to develop resistance in E. coli (ATCC 25922) as high above the minimum inhibitory concentration (MIC) as possible. Resistance was developed up to 2500 μg/mL of kanamycin, 100 times the MIC of 25 μg/mL. However, more frequently, resistance could be developed to 100 μg/mL. The antibacterial-resistant E. coli was then used in a cross-resistance study. This study aimed to find whether kanamycin resistance translated to resistance to other antibiotics. The kanamycin-resistant E. coli were tested against neomycin and gentamicin (both aminoglycosides) and ampicillin (a β-lactam). The kanamycin-resistance did not confer resistance to ampicillin, but resistance above the wild-type MIC was seen with gentamicin and to a larger extent with neomycin.
Daniel Marous (Advisor)
Ray Dudek (Committee Member)
Brian Yontz (Committee Member)
18 p.
Biochemistry; Chemistry; Microbiology; Molecular Biology
antibiotic resistance; antimicrobial resistance; AMR; E coli; kanamycin; neomycin; gentamicin; aminoglycosides; ampicillin; cross-resistance; beta-lactam antibiotics

Recommended Citations

Citations

  • Malott, B. (2019). Development and investigation of antibiotic resistance in E. coli using aminoglycosides [Undergraduate thesis, Wittenberg University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617617698561944

    APA Style (7th edition)

  • Malott, Bradley. Development and investigation of antibiotic resistance in E. coli using aminoglycosides. 2019. Wittenberg University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617617698561944.

    MLA Style (8th edition)

  • Malott, Bradley. "Development and investigation of antibiotic resistance in E. coli using aminoglycosides." Undergraduate thesis, Wittenberg University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=wuhonors1617617698561944

    Chicago Manual of Style (17th edition)

wuhonors1617617698561944
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© 2019, all rights reserved.
This open access ETD is published by Wittenberg University Honors Theses and OhioLINK.