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Full text release has been delayed at the author's request until January 16, 2026

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Studies of selective and reversible inhibitors of calcium entry in immune cells

Gandi, Naga Lakshmi
http://rave.ohiolink.edu/etdc/view?acc_num=wright1736679934429587

Abstract Details

2024, Master of Science (MS), Wright State University, Microbiology and Immunology.
Calcium signaling is a crucial regulator in many cellular processes, from immune response modulation to gene expression and apoptosis. Here, I have investigated the dynamics of calcium entry pathways, namely, store-operated calcium entry and store-independent calcium entry using a variety of cell types such as Jurkat T cells, HEK-293 cells and murine macrophages. Using fluorescence calcium imaging and selective inhibitor compounds, I have investigated the contributions of Orai and related calcium channels to calcium entry in both immune and non-immune cell types. The results demonstrate the pharmacological modulation of store-operated and store-independent calcium entry, showing the ability of compounds such as AE-19, AE-10, and EL-113 to inhibit calcium entry. Experiments involving membrane depolarization with elevated potassium concentrations confirmed that calcium entry through the plasma membrane is membrane potential-dependent. My results highlight the medical relevance of these channels, especially in the process of tubular aggregated myopathy, immune regulation and cancer. My studies may lead to therapeutics targeting calcium signaling pathways relevant to immune and muscular disorders.
J. Ashot Kozak, Ph. D. (Advisor)
Dawn Wooley, Ph.D. (Committee Member)
Marjorie M. Markopoulos, Ph.D. (Committee Member)
68 p.
Cellular Biology; Immunology; Microbiology; Neurosciences; Physiology
calcium signaling; store operated calcium channel; store independent calcium channel; calcium release activated calcium channel; Orai channels; Orai1; Orai2; Orai3; Fura-2AM imaging; Jurkat T cells; Hek cells; macrophages; depolarization

Recommended Citations

Citations

  • Gandi, N. L. (2024). Studies of selective and reversible inhibitors of calcium entry in immune cells [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1736679934429587

    APA Style (7th edition)

  • Gandi, Naga Lakshmi. Studies of selective and reversible inhibitors of calcium entry in immune cells. 2024. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1736679934429587.

    MLA Style (8th edition)

  • Gandi, Naga Lakshmi. "Studies of selective and reversible inhibitors of calcium entry in immune cells." Master's thesis, Wright State University, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=wright1736679934429587

    Chicago Manual of Style (17th edition)

wright1736679934429587
© 2024, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.
Release 3.2.12