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Full text release has been delayed at the author's request until May 06, 2025

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Evaluating Functional Inhibitors Of Acid Sphingomyelinase As Potential Negative Modulators Of Microvesicle Particle Release In Response To Skin Stressors

Sabit, Taskin
http://orcid.org/0009-0004-4950-2199
http://rave.ohiolink.edu/etdc/view?acc_num=wright1714664824381284

Abstract Details

2024, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
Many diseases including lupus erythematosus, atopic eczema, and rosacea can be associated with photosensitivity, which is an abnormal response to sunlight. Recent studies by our group have implicated microvesicle particles (MVP) as playing an important role in causing inflammation and systemic immunosuppression. Previous studies show that functional inhibitors of acid sphingomyelinase (FIASMA) can block MVP release in response to ultraviolet B (UVB) radiation or Platelet-activating Factor (PAF) agonist, which are skin stressors. However, more research needs to be conducted to evaluate the role of PAF, UVB and FIASMA in MVP generation and to formulate a therapeutic medication to be potentially used to treat photosensitivity. One of the major obstacles is the limited availability of human skin explants for research use. To investigate the mechanism of MVP generation and inhibition, we have proposed a preclinical model, porcine skin explants, as a substitute for human skin explants, which is comparatively easier to procure. In this study, we used one of five different FIASMA drugs imipramine, amitriptyline, desipramine, nortriptyline, and sertraline in combination with UVB-, PAF-or phorbol ester-mediated MVP release. This study suggests that FIASMA drugs can block MVP release in response to multiple skin stressors on human HaCaT keratinocyte cells, as well as human and porcine skin explants.
Jeffrey B. Travers, M.D., Ph.D. (Advisor)
Michael George Kemp, Ph.D. (Committee Member)
Ravi P. Sahu, Ph.D. (Committee Member)
73 p.
Immunology; Pharmacology; Toxicology
microvesicles; microvesicle particle; MVP; photosensitivity; FIASMA; Functional Inhibitors of Acid Sphingomyelinase

Recommended Citations

Citations

  • Sabit, T. (2024). Evaluating Functional Inhibitors Of Acid Sphingomyelinase As Potential Negative Modulators Of Microvesicle Particle Release In Response To Skin Stressors [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1714664824381284

    APA Style (7th edition)

  • Sabit, Taskin. Evaluating Functional Inhibitors Of Acid Sphingomyelinase As Potential Negative Modulators Of Microvesicle Particle Release In Response To Skin Stressors. 2024. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1714664824381284.

    MLA Style (8th edition)

  • Sabit, Taskin. "Evaluating Functional Inhibitors Of Acid Sphingomyelinase As Potential Negative Modulators Of Microvesicle Particle Release In Response To Skin Stressors." Master's thesis, Wright State University, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=wright1714664824381284

    Chicago Manual of Style (17th edition)

wright1714664824381284
© 2024, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.
Release 3.2.12