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Shikshita Singh-Thesis.pdf (1.13 MB)

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Porcine skin explants as a new model to investigate microvesicle particle generation

Singh, Shikshita
http://orcid.org/0009-0006-1188-697X
http://rave.ohiolink.edu/etdc/view?acc_num=wright1683900851572474

Abstract Details

2023, Master of Science (MS), Wright State University, Pharmacology and Toxicology.
The skin is exposed to a variety of external stressors, from irritants to toxic agents to ultraviolet radiation (UVR). While mice and human volunteers can be used for research studies, there are limitations in the range of environmental stressors that can be studied using these models. Therefore, our research team has been investigating the use of human skin explants as a model to study the immediate effects of environmental stressors ex vivo. Although this method has been successful, obtaining fresh human skin for these studies is difficult due to limited availability. To overcome this issue, we are evaluating the possibility of using porcine skin explants in our model systems. The primary advantage of using porcine skin is that it is readily available in large quantities, and its structure bears many similarities to that of human skin. In our research, we have found that various stressors, including UVB radiation, can trigger the production of Platelet-Activating Factor (PAF) in the skin. This, in turn, activates the enzyme acid sphingomyelinase (aSMase), leading to the shedding of subcellular microvesicle particles (MVP). In our research studies, we have used various models, such as cell lines, mice, human skin explants, and limited studies in human volunteers. Currently, we are examining the possibility of using porcine skin explants as a model to study MVP release in response to different types of stressors that are relevant to the skin. The application of the carbamyl PAF agonist PAF (CPAF) or a phorbol ester topically to the skin leads to an increase in MVP release. Similarly, exposing the skin to different UVB fluences also results in a higher release of MVP. Our experiments on porcine skin explants show similar results to human skin explants in terms of MVP release, although porcine skin appears to be less reactive to these agents. Although porcine skin shares many structural similarities with human skin, unlike human skin and cell lines, the combined impact of these stressors on porcine skin did not produce any synergistic results. Similar physiology does not necessarily imply that the skin will react to external stimuli in the same way. As the use of porcine skin in this type of skin model is relatively new, further research is needed to evaluate all parameters before considering porcine skin as a viable substitute for human skin models.
Jeffrey B. Travers, M.D., Ph.D. (Advisor)
David Cool, Ph.D. (Committee Member)
Yangfang Chen, M.D., Ph.D. (Committee Member)
55 p.
Pharmacology; Toxicology
microvesicle particle generation; porcine skin; human skin; UVB radiation; Platelet Activating Factor; acid sphingomyelinase; carbamyl PAF agonist; phorbol ester

Recommended Citations

Citations

  • Singh, S. (2023). Porcine skin explants as a new model to investigate microvesicle particle generation [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1683900851572474

    APA Style (7th edition)

  • Singh, Shikshita. Porcine skin explants as a new model to investigate microvesicle particle generation. 2023. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1683900851572474.

    MLA Style (8th edition)

  • Singh, Shikshita. "Porcine skin explants as a new model to investigate microvesicle particle generation." Master's thesis, Wright State University, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=wright1683900851572474

    Chicago Manual of Style (17th edition)

wright1683900851572474
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© 2023, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.