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Master's Thesis-Final.pdf (2.97 MB)

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Instability and Extrachromosomal Circular DNA Formation at Microsatellites and Unstable DNA Sequences

Shanahan, Matilyn M.
http://orcid.org/0000-0003-1718-0418
http://rave.ohiolink.edu/etdc/view?acc_num=wright1662034720827731

Abstract Details

2022, Master of Science (MS), Wright State University, Biochemistry and Molecular Biology.
We have previously documented our evidence of genetic instabilities at the (Pu/Py)78 and (ATTCT)47 sequences and our reasoning for identifying break-induced replication (BIR) as the mode of repair responsible for the mutations in the DNA flanking the unstable inserts. Now, as our lab investigates the protein mechanisms at play in the BIR pathway taking place at these sites, we are also expanding our knowledge of how this mechanism extends into the pathways responsible for forming extrachromosomal circular DNA (eccDNA) molecules. We have documented the phenomena posed as the driving factors for eccDNA formation in our systems containing (Pu/Py)78 and (ATTCT)47. Therefore, in this defense, we worked to uncover how STN1, COPS2, Polī¨ and, generally, BIR function to produce eccDNAs in mammalian cells containing these unstable DNA sequences using inverse PCR (iPCR) to detect circular DNAs being generated in the genomic contents of these cells before and after knockdown of the proteins of interest.
Michael Leffak, Ph.D. (Advisor)
Kwang-Jin Cho, Ph.D. (Committee Member)
Weiwen Long, Ph.D. (Committee Member)
122 p.
Biochemistry; Molecular Biology
extrachromosomal circular DNA (eccDNA); microsatellite DNAs; Break-induced replication; SN1; COPS2; Polymerase Eta; Inverse PCR (iPCR); DNA repair

Recommended Citations

Citations

  • Shanahan, M. M. (2022). Instability and Extrachromosomal Circular DNA Formation at Microsatellites and Unstable DNA Sequences [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1662034720827731

    APA Style (7th edition)

  • Shanahan, Matilyn. Instability and Extrachromosomal Circular DNA Formation at Microsatellites and Unstable DNA Sequences. 2022. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1662034720827731.

    MLA Style (8th edition)

  • Shanahan, Matilyn. "Instability and Extrachromosomal Circular DNA Formation at Microsatellites and Unstable DNA Sequences." Master's thesis, Wright State University, 2022. http://rave.ohiolink.edu/etdc/view?acc_num=wright1662034720827731

    Chicago Manual of Style (17th edition)

wright1662034720827731
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This open access ETD is published by Wright State University and OhioLINK.