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Transcriptional characterization of osteogenic and adipogenic differentiation of human bone marrow derived mesenchymal stem cells in 2D and 3D peptide hydrogel culture system.

Rony, R M Imtiaz Karim
http://rave.ohiolink.edu/etdc/view?acc_num=wright1536841298659517

Abstract Details

2018, Master of Science (MS), Wright State University, Biological Sciences.
Mesenchymal stem cells (MSCs) are adult multipotent stem cell that can differentiate into mesodermal lineages such osteoblast, adipocytes, and chondrocytes, or can be transdifferentiated into clinically relevant lineages such as cardiac or neural cells using in vitro reprogramming techniques. In addition to the multilineage differentiation potential, MSCs from most tissue origins such bone marrow derived mesenchymal stem cells (BM-MSCs) or adipose derived mesenchymal stem cells (AD-MSCs) have immune modulatory functions which indicate their promise in clinical applications for cell-based therapy, tissue engineering, and regenerative medicine. In recent years, three-dimensional (3D) cell culture systems, which utilize 3D scaffolds or hydrogels to provide a physiological 3D microenvironment that mimics the native extracellular matrix, have been largely embraced to study cellular function and cell fate determination using different cell types. Although many investigators have shown that stem cell self-renewal/differentiation is modulated by the 3D scaffold chemistry and/or its surface topology, less attention has been given to undercover how such 3D scaffold affects chromatin organization and gene expression of key developmental regulators or lineage specifiers at the transcription and translation level during the course of stem cell differentiation. In this novel study, we have established a Corning PuraMatrix Peptide Hydrogel based 3D culture system for BM-MSCs and have analyzed key transcription factors regulating osteogenic (RUNX2, OSX, TWIST1) and adipogenic (PPARy, C/EBPa, CHOP10) differentiation of BM-MSCs grown in a 2D culture and a PuraMatrix peptide hydrogel-based 3D culture. BM-MSCs adopt a completely different morphology and metabolism when encapsulated in PuraMatrix peptide hydrogel. Moreover, expression of these key osteogenic (RUNX2, OSX, TWIST1) and adipogenic (PPARy, C/EBPa, CHOP10) transcription factors are differentially expressed between 2D and 3D culture system at different stages of BM-MSCs differentiation.
Katherine Excoffon, Ph.D. (Committee Co-Chair)
Jaime E. Ramirez-Vick, Ph.D. (Committee Co-Chair)
Barbara Hull, Ph.D. (Committee Member)
Shulin Ju, Ph.D. (Committee Member)
113 p.
Biology; Biomedical Engineering; Biomedical Research; Cellular Biology; Developmental Biology
Stem Cells; Mesenchymal Stem Cells; Differentiation; 3D cell culture; Hydrogel; Tissue Engineering; Gene Expression; Regenerative Medicine

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Citations

  • Rony, R. M. I. K. (2018). Transcriptional characterization of osteogenic and adipogenic differentiation of human bone marrow derived mesenchymal stem cells in 2D and 3D peptide hydrogel culture system. [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1536841298659517

    APA Style (7th edition)

  • Rony, R M Imtiaz Karim. Transcriptional characterization of osteogenic and adipogenic differentiation of human bone marrow derived mesenchymal stem cells in 2D and 3D peptide hydrogel culture system. 2018. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1536841298659517.

    MLA Style (8th edition)

  • Rony, R M Imtiaz Karim. "Transcriptional characterization of osteogenic and adipogenic differentiation of human bone marrow derived mesenchymal stem cells in 2D and 3D peptide hydrogel culture system." Master's thesis, Wright State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=wright1536841298659517

    Chicago Manual of Style (17th edition)

wright1536841298659517
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© 2018, all rights reserved.
This open access ETD is published by Wright State University and OhioLINK.
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