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Thesis Erin Roberts FINAL.pdf (2.26 MB)

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Cytokine expression, cytoskeleton organization, and viability of SIM-A9 microglia exposed to Staphylococcus aureus-derived lipoteichoic acid and peptidoglycan

Roberts, Erin
http://rave.ohiolink.edu/etdc/view?acc_num=wright1515329731281897

Abstract Details

2017, Master of Science (MS), Wright State University, Microbiology and Immunology.
In these experiments, SIM-A9 microglia were exposed to Staphylococcus aureus cell wall components lipoteichoic acid (LTA) and peptidoglycan (PGN) at a concentration of 5 ug/mL for six, twelve, eighteen, and twenty-four hours and the cytokine expression, cytoskeleton organization, and cell viability of the cells were observed. Following LTA and PGN exposure, TNF-a; secretion increased at each time interval and was highest observed at 24 hours. No significant IL-10 secretion was detected. Over the 24 hour period, cell viability and cytotoxicity of LTA and PGN treatment groups were not significantly different from the control, indicating the observed inflammatory cytokine response was not due to cell death. These data suggest that LTA and PGN play a predominantly inflammatory role in the first twenty-four hours of S. aureus CNS infection.
Nancy Bigley, Ph.D. (Advisor)
Barbara Hull, Ph.D. (Committee Member)
Dawn Wooley, Ph.D. (Committee Member)
66 p.
Immunology; Microbiology
Staphylococcus aureus; lipoteichoic acid; LTA; peptidoglycan; PGN; microglia; CNS; TNFa; IL-10; tubulin; actin; viability; cytotoxicity;

Recommended Citations

Citations

  • Roberts, E. (2017). Cytokine expression, cytoskeleton organization, and viability of SIM-A9 microglia exposed to Staphylococcus aureus-derived lipoteichoic acid and peptidoglycan [Master's thesis, Wright State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=wright1515329731281897

    APA Style (7th edition)

  • Roberts, Erin. Cytokine expression, cytoskeleton organization, and viability of SIM-A9 microglia exposed to Staphylococcus aureus-derived lipoteichoic acid and peptidoglycan. 2017. Wright State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=wright1515329731281897.

    MLA Style (8th edition)

  • Roberts, Erin. "Cytokine expression, cytoskeleton organization, and viability of SIM-A9 microglia exposed to Staphylococcus aureus-derived lipoteichoic acid and peptidoglycan." Master's thesis, Wright State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright1515329731281897

    Chicago Manual of Style (17th edition)

wright1515329731281897
559
© 2017, some rights reserved.Creative Commons License Cytokine expression, cytoskeleton organization, and viability of SIM-A9 microglia exposed to Staphylococcus aureus-derived lipoteichoic acid and peptidoglycan by Erin Roberts is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Wright State University and OhioLINK.