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INVESTIGATION OF THE ROLE OF ANNEXIN V IN MOUSE PLACENTA: DEVELOPMENT OF APPROACHES TO EXPLORE THE THERAPEUTIC POTENTIAL OF THE PROTIEN

Wang, Xiuqiong
http://rave.ohiolink.edu/etdc/view?acc_num=ucin968165255

Abstract Details

2000, PhD, University of Cincinnati, Medicine : Molecular and Cellular Physiology.
Annexin V is a promising candidate as an anticoagulant and anti-inflammatory agent due to its high affinity for anionic phospholipid surfaces. Its abundance and tissue localization in placental trophoblasts and vascular endothelial cells imply a physiological importance in these tissues. In the first section of this study, I demonstrate that annexin V is critical in maintaining murine placental integrity. I conclude that the protecting role of annexin V appears to be local because I did not detect the protein in culture media of either trophoblasts or endothelial cells. In the next portion of this study, I successfully targeted annexin V to the secretory pathway of mammalian cells and the protein was secreted into the medium. This unnatural targeting of annexin V to the lumen of the endoplasmic reticulum did not have significant effect on cellular function. Therefore, I concluded that targeting secretion of annexin V in transgenic animals should provide a valuable model for evaluating the therapeutic potentials of annexin V as anticoagulant and/or anti-inflammatory agent.
John Dedman (Advisor)
120 p.
Biology, Molecular
Protein Secretion; Immunofluorescence; Calcium Homeostensis; Transgenic Mice; Pregnancy Loss

Recommended Citations

Citations

  • Wang, X. (2000). INVESTIGATION OF THE ROLE OF ANNEXIN V IN MOUSE PLACENTA: DEVELOPMENT OF APPROACHES TO EXPLORE THE THERAPEUTIC POTENTIAL OF THE PROTIEN [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin968165255

    APA Style (7th edition)

  • Wang, Xiuqiong. INVESTIGATION OF THE ROLE OF ANNEXIN V IN MOUSE PLACENTA: DEVELOPMENT OF APPROACHES TO EXPLORE THE THERAPEUTIC POTENTIAL OF THE PROTIEN. 2000. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin968165255.

    MLA Style (8th edition)

  • Wang, Xiuqiong. "INVESTIGATION OF THE ROLE OF ANNEXIN V IN MOUSE PLACENTA: DEVELOPMENT OF APPROACHES TO EXPLORE THE THERAPEUTIC POTENTIAL OF THE PROTIEN." Doctoral dissertation, University of Cincinnati, 2000. http://rave.ohiolink.edu/etdc/view?acc_num=ucin968165255

    Chicago Manual of Style (17th edition)

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© 2000, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.
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