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Development and Optimization of a Cell Derived Matrix Assay using Primary Lung Fibroblasts

Adams, Morgan
http://rave.ohiolink.edu/etdc/view?acc_num=ucin172139732999051

Abstract Details

2024, MS, University of Cincinnati, Medicine: Biomedical Research Technology.
The ECM plays an important role in the structure, function and signaling within tissues. Changes to the ECM can cause long-lasting alterations that have been seen to influence disease progression. In vivo models currently lack the technological capabilities to clearly visualize the ECM. The inability to study the ECM and monitor changes to the ECM in vivo has created a need to use in vitro models to learn more about the ECM. Using in vitro models in combination with in vivo models’ researchers can mimic the in vivo ECM to visualize and study changes that occur within the ECM. Of the current in vitro models, a cell-derived matrix (CDM) assay can be used to study the composition, structure and cell-matrix interactions. Use of this model can show how the ECM changes in response to injury and disease. In this project, we adapted and troubleshooted a CDM assay published by Franco-Barraza et al., in 2017 to work with primary lung fibroblasts. We worked out the best way to allow cells to attach to the plate, grow, and then secrete matrix. Using confocal microscopy we evaluated the deposited ECM protein fibronectin. We worked out the protocol to work that works with murine primary lung fibroblasts. We can now use this adapted protocol for further testing of ECM protein secretion and studying how other cells use the produced matrix.
Anne-Karina Perl, Ph.D. (Committee Chair)
Jason Tchieu, Ph.D. (Committee Member)
Kristin Hudock (Committee Member)
14 p.
Biology
Extracellular Matrix; Fibronectin; Primary Lung Fibroblasts; Cell Derived Matrix

Recommended Citations

Citations

  • Adams, M. (2024). Development and Optimization of a Cell Derived Matrix Assay using Primary Lung Fibroblasts [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin172139732999051

    APA Style (7th edition)

  • Adams, Morgan. Development and Optimization of a Cell Derived Matrix Assay using Primary Lung Fibroblasts. 2024. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin172139732999051.

    MLA Style (8th edition)

  • Adams, Morgan. "Development and Optimization of a Cell Derived Matrix Assay using Primary Lung Fibroblasts." Master's thesis, University of Cincinnati, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=ucin172139732999051

    Chicago Manual of Style (17th edition)

ucin172139732999051
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This open access ETD is published by University of Cincinnati and OhioLINK.