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Continuous Measurement of Sensory Responses in a 3-Dimensional Dermal Substitute for Neuropathic Research

Klug, Justin
http://orcid.org/0009-0009-2193-9096
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1721232587450373

Abstract Details

2024, MS, University of Cincinnati, Engineering and Applied Science: Biomedical Engineering.
Peripheral neuropathy is a diverse affliction caused by numerous forms of traumatic injuries and genetic diseases. Damage or mutation in the peripheral nervous system (PNS) can result in unpleasant alterations to normal sensation, such as chronic itch or chronic pain. There is no cure for peripheral neuropathy and research models of the PNS are limited due to its vital nature in an organism and its grand scale. Only in-vitro and in-utero models allow for observing interactions between the distal and proximal ends of sensory neurons (SNs) in tandem. Current three-dimensional (3D) in-vitro models enable simulating the PNS in different tissue environments, such as the skin. However, current innervated models are limited in options for measuring action potentials of SNs in the 3D environment. Such models use non-repeatable measurements or costly experimental measuring apparatuses, making processes that change over time, such as healing or degradation, a significant challenge to observe. In this article we describe the creation of an innervated dermis optimized to provide surface access to SNs so individual neuron action potentials can be clearly distinguished from each other while still achieving transdermic sensation for use with calcium imaging. Next, we develop our own method for repeatably measuring action potentials using our model that provides continuous measurement of action potentials in a non-destructive manner, without the need for a customized imaging apparatus. Our new model can simultaneously measure multiple action potentials from dozens of neurons numerically and continuously across multiple days.
Stacey Schutte, Ph.D. (Committee Chair)
Eric Nauman, Ph.D. (Committee Member)
Greg Harris, Ph.D. (Committee Member)
28 p.
Biomedical Engineering
Neuron; skin; tissue engineering; innervation

Recommended Citations

Citations

  • Klug, J. (2024). Continuous Measurement of Sensory Responses in a 3-Dimensional Dermal Substitute for Neuropathic Research [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1721232587450373

    APA Style (7th edition)

  • Klug, Justin. Continuous Measurement of Sensory Responses in a 3-Dimensional Dermal Substitute for Neuropathic Research. 2024. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1721232587450373.

    MLA Style (8th edition)

  • Klug, Justin. "Continuous Measurement of Sensory Responses in a 3-Dimensional Dermal Substitute for Neuropathic Research." Master's thesis, University of Cincinnati, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1721232587450373

    Chicago Manual of Style (17th edition)

ucin1721232587450373
18
© 2024, some rights reserved.Creative Commons License Continuous Measurement of Sensory Responses in a 3-Dimensional Dermal Substitute for Neuropathic Research by Justin Klug is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.