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Microdissection of the bone marrow microenvironment

Patel, Pranjal
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1712916838567666

Abstract Details

2024, MS, University of Cincinnati, Medicine: Immunology.
Bone marrow has the most mysterious anatomy of differentiation compared to the vast majority of other tissues. In fact, assessing blood cell production in situ is difficult due to a lack of strategies to examine differentiation, lineage commitment decisions, and tissue functionality with regard to spatial organizing cues. All blood cells originate from hematopoietic stem cells (HSCs). The bone marrow microenvironment contains HSCs and non-hematopoietic cells where they form a niche which directs hematopoietic output. At times of homeostasis or stress, microenvironmental cues direct HSCs to differentiate and proliferate and these hematopoietic niches are commonly referred to as HSC niches. It may be possible to develop novel therapeutic approaches for HSCs and their progeny, which are affected by microenvironment dysfunction. By using cold active protease generated from Bacillus licheniformis, we hope to understand the microenvironment through microdissection and sequencing techniques. Previous studies have shown that single cell RNA sequencing artifacts are significantly reduced compared to conventional methods [1]. Our method enables us to isolate vasculature cells from mouse sternums via microdissection, without using commonly used fixation steps for tissue imaging. Through this optimized technique, downstream analysis, such as scRNAseq of the dissected area can be performed to understand both the steady state as well as the diseased microenvironment of bone marrow.
Jonathan Katz, Ph.D. (Committee Chair)
Ian Lewkowich, Ph.D. (Committee Member)
Daniel Lucas, Ph.D. (Committee Member)
21 p.
Immunology
Bone marrow; HSCs; microdissection; microenviornment; niche; Bacillus licheniformis

Recommended Citations

Citations

  • Patel, P. (2024). Microdissection of the bone marrow microenvironment [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1712916838567666

    APA Style (7th edition)

  • Patel, Pranjal. Microdissection of the bone marrow microenvironment. 2024. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1712916838567666.

    MLA Style (8th edition)

  • Patel, Pranjal. "Microdissection of the bone marrow microenvironment." Master's thesis, University of Cincinnati, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1712916838567666

    Chicago Manual of Style (17th edition)

ucin1712916838567666
52
© 2024, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.
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