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Full text release has been delayed at the author's request until April 25, 2026

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Electroosmotic flow driven free antigens reaction with immobilized magnetic-microbeads-tagged-antibodies in microchannel

Ajiboye, Israel
http://orcid.org/0009-0005-3833-7463
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1712914132044215

Abstract Details

2024, MS, University of Cincinnati, Engineering and Applied Science: Mechanical Engineering.
Immunoassays based on reactions between target pathogen (antigen; Ag) and antibody (Ab) are frequently used for Ag detection. In this study, an external magnetic field was used to immobilize magnetic microbeads-tagged-antibodies (mMB-Ab) on the surface of a microchannel in the capture zone. The mMB-Ab was subsequently used for Ag detection. The objective of this study is to numerically assess the surface reaction between mMB-Ab and Ag in the presence of electroosmotic flow (EOF). First, mMB-Ab complex was immobilized in the wall of the capture zone. Subsequently, the Ag was transported by EOF towards the capture zone to bind with the immobilized mMB-Ab. Lastly, mMB-Ab:Ag complex was formed and immobilized in the capture zone. A finite volume solver was used to implement the above steps. The surface reaction between the mMB-Ab and Ag was investigated in the presence of electric fields (E): 150 V/cm - 450 V/cm and Ag concentrations: 0.001 M - 1000 M. The mMB-Ab got depleted at t = 0.2 s, 0.4 s, and 0.8 s for an E of 450 V/cm, 275 V/cm, and 150 V/cm, respectively. The depletion of mMB-Ab increases with time as the E decreases. This is because a decrease in the E results in a reduction of velocity caused by EOF, leading to a delay in time for depletion. Furthermore, as the concentration of Ag decreases, the depletion of mMB-Ab increases with time. Therefore, the flow and concentration of Ag influenced the surface reaction. These results quantify the detection of Ag using the EOF device; thus, highlighting its potential for rapid throughput screening of Ag. This platform technology can lead to the development of a portable device for the detection of target cells, pathogens, and biomolecules, which can be used for testing water systems, biological fluids, and biochemicals.
Rupak Banerjee, Ph.D (Committee Chair)
Kishan Bellur, Ph.D. (Committee Member)
Je-Hyeong Bahk, Ph.D. (Committee Member)
58 p.
Biomedical Research
Electroosmotic flow; antibody; antigen; magnetic micobeads; surface reaction; microchannel

Recommended Citations

Citations

  • Ajiboye, I. (2024). Electroosmotic flow driven free antigens reaction with immobilized magnetic-microbeads-tagged-antibodies in microchannel [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1712914132044215

    APA Style (7th edition)

  • Ajiboye, Israel. Electroosmotic flow driven free antigens reaction with immobilized magnetic-microbeads-tagged-antibodies in microchannel. 2024. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1712914132044215.

    MLA Style (8th edition)

  • Ajiboye, Israel. "Electroosmotic flow driven free antigens reaction with immobilized magnetic-microbeads-tagged-antibodies in microchannel." Master's thesis, University of Cincinnati, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1712914132044215

    Chicago Manual of Style (17th edition)

ucin1712914132044215
© 2023, some rights reserved.Creative Commons License Electroosmotic flow driven free antigens reaction with immobilized magnetic-microbeads-tagged-antibodies in microchannel by Israel Ajiboye is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.
Release 3.2.12