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Generation of an Acute Myeloid Leukemia Mouse Model by Loss of DNA-pk and DEK

Shephard, Miranda
http://orcid.org/0000-0002-4800-1577
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1668635271288988

Abstract Details

2022, MS, University of Cincinnati, Medicine: Biomedical Research Technology.
The majority of acute myeloid leukemia (AML) cases have chromosomal abnormalities, composed of translocations or aneuploidies. The oncogene DEK was first identified in acute myeloid leukemia as a translocation with NUP214. However, the role of full length DEK in AML is poorly understood. DEK does have a role in DNA repair pathways, including Homologous Recombination and Non-homologous End-Joining, where it promotes DNA-PK activity. This study investigates the development of a DNA-pk mutant and DEK-deficient mouse model. In this novel mouse model, tissue histology, along with blood and bone marrow immunophenotyping, determined that dual loss of DNA-pk and DEK generated a murine model of leukemia. DNA-pk mutant and DEK deficient mice exhibited a reduction in early hematopoietic stem and multipotent progenitor cells while also displaying an increase in the number of granulocyte-monocyte progenitors and differentiated granulocytes and neutrophils. Generation of a mouse model with dual loss of DEK and DNA-pk will serve as a tool to further investigate the role of full length DEK in leukemia.
Lisa Vinnedge, Ph.D. (Committee Member)
Jason Tchieu, Ph.D. (Committee Member)
Marie-Dominique Filippi, Ph.D. (Committee Member)
16 p.
Oncology
DEK; DNA-pk; leukemia; mouse; blood; bone marrow

Recommended Citations

Citations

  • Shephard, M. (2022). Generation of an Acute Myeloid Leukemia Mouse Model by Loss of DNA-pk and DEK [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1668635271288988

    APA Style (7th edition)

  • Shephard, Miranda. Generation of an Acute Myeloid Leukemia Mouse Model by Loss of DNA-pk and DEK. 2022. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1668635271288988.

    MLA Style (8th edition)

  • Shephard, Miranda. "Generation of an Acute Myeloid Leukemia Mouse Model by Loss of DNA-pk and DEK." Master's thesis, University of Cincinnati, 2022. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1668635271288988

    Chicago Manual of Style (17th edition)

ucin1668635271288988
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© 2022, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.
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