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34197.pdf (1.18 MB)
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Regulation Of Hematopoietic Stem Cells By Lipid and Mitochondrial Metabolism
Author Info
Sharma, Devyani
ORCID® Identifier
http://orcid.org/0000-0001-8301-8756
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1563295190003946
Abstract Details
Year and Degree
2020, MS, University of Cincinnati, Medicine: Biomedical Research Technology.
Abstract
Hematopoietic Stem Cells (HSC) are found in the bone marrow niche of the human body. HSC are the only cells that have both multi-lineage differentiation and self-renewal potential. Maintenance of a healthy HSC pool is crucial for a successful stem cell transplant, which represents a curative therapy for the treatment of hematological disorders. It is well known that with age HSCs lose their ability to differentiate into the various blood lineages. Previous data from our lab has shown that HSCs from the aged animals or after bone marrow transplantation exhibit an altered mitochondrial morphology. The objective of this project is to understand the mechanism of aging of the HSCs and to reverse this effect in order to improve HSC regeneration. Our results show that mitochondrial morphology and its membrane potential are necessary to maintain the differentiation capability of the HSCs. Old HSC have reduced cardiolipin content, a lipid important for mitochondrial functions. Finally, lipid supplementation improves the mitochondrial membrane potential and improves mitochondrial morphology. Together, these findings suggest that lipid supplements may improve HSC functions, which could be used during bone marrow transplantation to promote HSC regenerative potential.
Committee
Rashmi Hegde, Ph.D. (Committee Chair)
Marie-Dominique Filippi, Ph.D. (Committee Member)
Damien Reynaud (Committee Member)
Pages
16 p.
Subject Headings
Aging
Keywords
Hematopoietic Stem Cells
;
Aging
;
Lipids
;
Mitochondria
;
Cardiolipin
;
LSK-SLAM
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Citations
Sharma, D. (2020).
Regulation Of Hematopoietic Stem Cells By Lipid and Mitochondrial Metabolism
[Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1563295190003946
APA Style (7th edition)
Sharma, Devyani.
Regulation Of Hematopoietic Stem Cells By Lipid and Mitochondrial Metabolism.
2020. University of Cincinnati, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1563295190003946.
MLA Style (8th edition)
Sharma, Devyani. "Regulation Of Hematopoietic Stem Cells By Lipid and Mitochondrial Metabolism." Master's thesis, University of Cincinnati, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1563295190003946
Chicago Manual of Style (17th edition)
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Document number:
ucin1563295190003946
Download Count:
122
Copyright Info
© 2019, some rights reserved.
Regulation Of Hematopoietic Stem Cells By Lipid and Mitochondrial Metabolism by Devyani Sharma is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.