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12799.pdf (3.56 MB)
ETD Abstract Container
Abstract Header
Thermo-Responsive Polymers for Cell-Based Therapeutic Applications
Author Info
James, Hodari-Sadiki L
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416231680
Abstract Details
Year and Degree
2014, MS, University of Cincinnati, Engineering and Applied Science: Biomedical Engineering.
Abstract
Poly (N-isopropylacrylamide) (PNIPAAm) is a well-known thermo-responsive polymer that has be shown to be biocompatible, with surfaces coated with PNIPAAm supporting the culture of cells. These surfaces support the adhesion and proliferation of multiple cell phenotypes at 37 °C, when surface is hydrophobic, as the polymer chains are collapse and lose their affinity for water. Reducing the temperature below the polymers lower critical solution temperature (LCST) elicits hydration and swelling of the polymer chains and leads to cell detachment. In vitro culture on thermo-responsive surfaces can be used to produce cell sheets for the use of different therapeutic treatments. PNIPAAm coated membranes were used to culture human keratinocyte cells to confluence, with cell release possible after exposing the membranes to room temperature (25 °C) for 10 minutes. Cell sheet transfer was possible from the coated membrane to cell culture dishes using a protocol that we developed. There was also a trend towards similar cell apoptosis on both PNIPAAm coated and uncoated surfaces.
Committee
Daria Narmoneva, Ph.D. (Committee Chair)
T. Douglas Mast, Ph.D. (Committee Member)
Dale Schaefer, Ph.D. (Committee Member)
Pages
41 p.
Subject Headings
Biomedical Research
Keywords
thermoresponsive
;
Poly N isopropylacrylamide
;
cell culture
;
keratinocytes
;
PNIPAAm
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Citations
James, H.-S. L. (2014).
Thermo-Responsive Polymers for Cell-Based Therapeutic Applications
[Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416231680
APA Style (7th edition)
James, Hodari-Sadiki.
Thermo-Responsive Polymers for Cell-Based Therapeutic Applications.
2014. University of Cincinnati, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416231680.
MLA Style (8th edition)
James, Hodari-Sadiki. "Thermo-Responsive Polymers for Cell-Based Therapeutic Applications." Master's thesis, University of Cincinnati, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1416231680
Chicago Manual of Style (17th edition)
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Document number:
ucin1416231680
Download Count:
728
Copyright Info
© 2014, some rights reserved.
Thermo-Responsive Polymers for Cell-Based Therapeutic Applications by Hodari-Sadiki L James is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by University of Cincinnati and OhioLINK.