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Role of SPDEF in Prostate Cancer

Gao, Chen
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1343051932

Abstract Details

2012, MS, University of Cincinnati, Medicine: Molecular and Developmental Biology.
SAM Pointed Domain Containing ETS Transcription Factor (SPDEF) is a new member of ETS transcription factor family that is originally discovered in the epithelium of prostate. Like other ETS family members, SPDEF is involved in multiple biological processes including cell fate determination and specification, cell proliferation, epithelial-to-mesenchymal transformation and migration. Although much work has been done to elucidate the role of SPDEF in normal physiological conditions and in cancers, its expression level in cancer is still controversial. Similarly, there is no conclusive message on the role of SPDEF in cancer despite the fact that several in vitro studies have been done. We first overviewed the discovery and properties of SPDEF, followed by a comprehensive review of the role of SPDEF, with a special focus on its role in the development of cancer. In the second chapter, we utilized a xenograft model to manipulate SPDEF level in mouse prostate cancer. After orthotopic injection, we found that overexpression of SPDEF resulted in smaller tumors in mice. We also showed that several target genes related to cell proliferation and migration are down-regulated by SPDEF overexpression. We confirmed down-regulation of these genes in prostate cancer cell lines. In fact, we found that SPDEF overexpression inhibited cell migration, resulting in reduced aggressiveness of tumor cells. In addition, SPDEF overexpression was shown to suppress tumor growth via alteration of cell cycle profile. Collectively, these data indicate that SPDEF suppresses tumor growth via inhibition of cell proliferation and migration.
Tanya Kalin, PhD (Committee Chair)
Vladimir Kalinichenko, MD PhD (Committee Member)
Jeffrey Whitsett, MD (Committee Member)
55 p.
Molecular Biology
SPDEF; prostate cancer; cell proliferation; cell migration; ; ;

Recommended Citations

Citations

  • Gao, C. (2012). Role of SPDEF in Prostate Cancer [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1343051932

    APA Style (7th edition)

  • Gao, Chen. Role of SPDEF in Prostate Cancer. 2012. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1343051932.

    MLA Style (8th edition)

  • Gao, Chen. "Role of SPDEF in Prostate Cancer." Master's thesis, University of Cincinnati, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1343051932

    Chicago Manual of Style (17th edition)

ucin1343051932
841
© 2012, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.