Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


ucin1304692634.pdf (327.28 KB)

ETD Abstract Container

Abstract Header

Novel Protein Materials based on Bacterial Efflux Pumps

Li, Dan
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1304692634

Abstract Details

2011, MS, University of Cincinnati, Engineering and Applied Science: Biomedical Engineering.
Artificial molecular motors have been an emerging research field in the last two decades. In this project we designed a novel functional vesicle system on the basis of a unique motor protein, the efflux transporter AcrB, and a photosensitive proton pump Bacteriorhodopsin (Br). Protein-containing lipid vesicle formation is a self-assembly process, with size distribution being verified by dynamic light scattering experiments. Proteins are incorporated into the lipid membrane to form a functional system that converts external photon energy into a proton motive force which is used by AcrB to actively pump substrates into the vesicle interior. The pumping performance of the designed vesicle system is quantitatively evaluated using Ethidium Bromide and the antibiotic substrate Bocillin. The active pumping rate is 0.01nmol/mg/s, which is comparable to current data from bacterial cell in vivo experiments. The addition of intravesicular nanodiamonds and linking AcrB with Br will further enhance the pumping ability and capacity of the total system, while adding an AcrB blocker will disable the pump and serve as a negative control. Furthermore, a theoretical model is constructed that can explain the different pH gradients for four types of vesicle systems and estimate the functionality of the system from the aspect of thermodynamics. The designed vesicle system is of great use for bioremediation, environmental health etc, and provides us with some useful clues for the further advancement of molecular machinery.
David Wendell, PhD (Committee Chair)
Dionysios Dionysiou, PhD (Committee Member)
Andrew Herr, PhD (Committee Member)
45 p.
Biomedical Research
Vesicle; Efflux transporter; Proton pump; Bioremediation

Recommended Citations

Citations

  • Li, D. (2011). Novel Protein Materials based on Bacterial Efflux Pumps [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1304692634

    APA Style (7th edition)

  • Li, Dan. Novel Protein Materials based on Bacterial Efflux Pumps. 2011. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1304692634.

    MLA Style (8th edition)

  • Li, Dan. "Novel Protein Materials based on Bacterial Efflux Pumps." Master's thesis, University of Cincinnati, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1304692634

    Chicago Manual of Style (17th edition)

ucin1304692634
421
© 2011, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.