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Girish Kumar ucin1225816129.pdf (46.54 MB)

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Cell Engineering: Regulating Cell Behaviors Using Micropatterned Biomaterials

Kumar, Girish
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1225816129

Abstract Details

2008, PhD, University of Cincinnati, Engineering : Chemical Engineering.
Controlling the spatial organization of cells is a critical step towards engineering tissues with distributed networks of blood vessels or nerve cells. Here we report a new soft-lithography based approach for micropatterning proteins and cells on the surface of biodegradable chitosan substrates that are more applicable to engineering tissues than the gold, silver, silicone, glass, or silicone substrates currently used in cell micropatterning studies. In this approach, we use random copolymers of oligoethyleneglycol methacrylate (OEGMA), which resists protein and cell adsorption, and methacrylic acid (MA), which adheres strongly onto chitosan substrate via acid-base interactions, to form stable protein and cell resistant micropatterns on chitosan surfaces. We have also developed a non-invasive in vitro migration assay that operates through release of confluent groups of cells initially confined within patterns of cell-resistant polyelectrolyte. Cell-resistant patterns of polyelectrolyte, separating groups of confluent cells, are rendered cell-adhesive by adsorption of a second, cell adhesive polyelectrolyte of opposite charge; thereby, resulting in migration of cells into the separating regions. By dynamically controlling cell-surface interactions through self-assembly of cell-adhesive and cell resistant polyelectrolytes, this method eliminates the need to mechanically wound cells, as is done in current cell migration assays. We used this technique in identifying molecules and mechanisms that regulate cell migration is demonstrated by its application as an assay for the effects of platelet derived growth factors, cytoskeleton disrupting agents, and Merlin over-expression, on the migration of NIH 3T3 fibroblasts. We further used our patterning technique to precisely control the directional migration of a cell. Directional control of cell migration is essential for engineering tissues with defined cellular architectures, promoting wound healing, and non-invasively manipulating cells in vitro. We have used a new approach, Microarray Amplification of Natural Directional Persistence (MANDIP), to guide the long-range directional migration of attached mammalian cells. Amplification of directional persistence occurs through the asymmetric positioning of microarray islands and restriction of lamellipodia attachment, and thus migration, to one preset direction. This new approach for precisely controlling directional migration of cells was used to explore the effects of RhoGTPases on directional migration of cells and applied to separate cell mixtures
Chia-Chi Ho, PhD (Advisor)
Carlos Co, PhD (Committee Member)
Ian Papautsky, PhD (Committee Member)
Stephen Clarson, PhD (Committee Member)
218 p.
Biomedical Research; Chemical Engineering
cell patterning; cell migration; soft lithography

Recommended Citations

Citations

  • Kumar, G. (2008). Cell Engineering: Regulating Cell Behaviors Using Micropatterned Biomaterials [Doctoral dissertation, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1225816129

    APA Style (7th edition)

  • Kumar, Girish. Cell Engineering: Regulating Cell Behaviors Using Micropatterned Biomaterials. 2008. University of Cincinnati, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1225816129.

    MLA Style (8th edition)

  • Kumar, Girish. "Cell Engineering: Regulating Cell Behaviors Using Micropatterned Biomaterials." Doctoral dissertation, University of Cincinnati, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1225816129

    Chicago Manual of Style (17th edition)

ucin1225816129
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© 2008, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.