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DESIGN PARAMETERS FOR TISSUE ENGINEERED IMPLANTS FOR RABBIT PATELLAR TENDON AND ACHILLES TENDON REPAIRS

JUNCOSA, LAURA NATALIA
http://rave.ohiolink.edu/etdc/view?acc_num=ucin1022080022

Abstract Details

2002, MS, University of Cincinnati, Engineering : Aerospace Engineering.
Previous work performed in Noyes-Giannetras Laboratory has shown that mechanical alignment of undifferentiated mesenchymal stem cells about a suture causes alignment of cells and contraction of constructs in culture in a form that is suitable for implantation for tendon repair. With preliminary proof of concept, it is now our goal to fine-tune this procedure to determine the various factors that will lead to the highest quality tissue from a biomechanical standpoint and the fastest cell proliferation rates in culture. The basis for this step assumes that natural in-vivo conditions are optimal for in-vitro culture and that if we can simulate in-vivo forces or strains for a variety of activities we can precondition the implant and cells to the signals they will receive after surgery. However, these in-vivo force patterns are generally not known for a tissue for different activities and likely vary from tendon to tendon. Knowing tendon and ligament forces during normal activities is important in order to understand the levels of in-vivo forces the constructs will be expected to bear once implanted. While researchers have to date failed to develop tissue-engineered replacements that match the ultimate mechanical properties of normal tissues, it is conceivable that less stringent design requirements based on normal activity forces (rather than ultimate or failure properties) may be sufficient for functional efficacy. The purpose of this research study was to determine the in-vivo force-time patterns acting on the rabbit patellar tendon and Achilles tendon models for two speeds of activity and for two inclinations of activity. In addition, we sought to determine the failure properties of these tissues so as to compute safety factors (i.e. ratios of failure tissue force to in-vivo operating force). This data will provide design parameters for preparing tissue engineered implants containing mesenchymal stem cells (MSCs) that will more effectively repair surgical defects in these same tissues. Eight rabbit patellar tendons (PT) and eight rabbit Achilles tendons (AT) were instrumented using implantable force transducers (IFTs), and each rabbit was subjected to an experimental design involving five activity levels. Peak tensile forces and rates of rise and fall in tendon force increased significantly with increasing activity (p<0.001). These data will be employed to mechanically stimulate tissue engineered implants in culture.
Dr. David L. Butler (Advisor)
77 p.
tissue engineering; patellar tendon; Achilles Tendon; implantable force transducer; rabbit model

Recommended Citations

Citations

  • JUNCOSA, L. N. (2002). DESIGN PARAMETERS FOR TISSUE ENGINEERED IMPLANTS FOR RABBIT PATELLAR TENDON AND ACHILLES TENDON REPAIRS [Master's thesis, University of Cincinnati]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1022080022

    APA Style (7th edition)

  • JUNCOSA, LAURA. DESIGN PARAMETERS FOR TISSUE ENGINEERED IMPLANTS FOR RABBIT PATELLAR TENDON AND ACHILLES TENDON REPAIRS. 2002. University of Cincinnati, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ucin1022080022.

    MLA Style (8th edition)

  • JUNCOSA, LAURA. "DESIGN PARAMETERS FOR TISSUE ENGINEERED IMPLANTS FOR RABBIT PATELLAR TENDON AND ACHILLES TENDON REPAIRS." Master's thesis, University of Cincinnati, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1022080022

    Chicago Manual of Style (17th edition)

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© 2002, all rights reserved.
This open access ETD is published by University of Cincinnati and OhioLINK.