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Applications of Mass Spectrometry for Qualitative Analysis and Imaging of Microcystins in Mouse Tissues, Cyanobacterial Cells and Water

Kucheriavaia, Daria
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1596824964059216

Abstract Details

2020, Master of Science, University of Toledo, Chemistry.
Microcystins (MCs) are widespread cyanotoxins present in water sources contaminated with cyanobacteria during harmful algal blooms (HABs), and are compounds of concern due to their hepatotoxicity. Despite the regulation of their allowed concentration in drinking water, humans could be exposed to MCs by water or food consumption, or during recreational activities in affected bodies of water. Therefore, the studies of mechanisms of MC activity, transportation and detoxification pathways are important for the better understanding of both their chronic and acute toxicity. Also, quick and reliable methods of water analysis are needed in order to determine the presence of the toxins and prevent human exposure. Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is a valuable technique for the visualization of analytes in biological tissues and other media. Herein, MALDI-MSI was applied to investigate the distribution of MC-LR and its metabolites in liver tissue sections obtained from mice exposed to MC-LR. The study was directed on the effect of the chronic MC-LR toxicity below the no observed adverse effect level (NOAEL) on control mice and mice used to model non-alcoholic fatty liver disease (NAFLD). Limits of detection (LOD) of MC-LR with MALDI-MS were determined for on-plate and on-tissue MALDI-MSI experiments. MALDI-MS imaging procedures were also optimized. Different variations of the matrix deposition and sample preparation were examined, and the most effective MALDI-MSI protocol was used for imaging of MC-LR in the liver tissue of the studied mice. MCs are produced within cyanobacterial cells, and their release into water could happen during cell death or water treatment procedures. Thus, cell lysis and MC release and extraction have to be studied and evaluated. At the same time, monitoring of the extracellular MCs in the water sources is an important task and should be rapid and reliable. In the second project, high-performance liquid chromatography mass spectrometry (HPLC-MS) and MALDI-MS were used for rapid determination of MC congeners in Lake Erie water collected during 2019 HAB. Three cell lysis and MC extraction methods were evaluated, and their influence on the toxin content was determined by HPLC and MALDI-MS. Overall, the potential of MALDI-MSI for the localization of microcystins in the liver tissue sections was shown. Additionally, HPLC-MS and MALDI-MS were applied for the qualitative analyses of MCs in cyanobacterial cells and environmental water samples originating from Lake Erie.
Dragan Isailovic (Committee Chair)
Eric Findsen (Committee Member)
Terry Bigioni (Committee Member)
132 p.
Analytical Chemistry; Chemistry
Microcystins; MALDI-MSI; imaging; HPLC-ESI-MS; orbitrap; HABs

Recommended Citations

Citations

  • Kucheriavaia, D. (2020). Applications of Mass Spectrometry for Qualitative Analysis and Imaging of Microcystins in Mouse Tissues, Cyanobacterial Cells and Water [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1596824964059216

    APA Style (7th edition)

  • Kucheriavaia, Daria. Applications of Mass Spectrometry for Qualitative Analysis and Imaging of Microcystins in Mouse Tissues, Cyanobacterial Cells and Water. 2020. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1596824964059216.

    MLA Style (8th edition)

  • Kucheriavaia, Daria. "Applications of Mass Spectrometry for Qualitative Analysis and Imaging of Microcystins in Mouse Tissues, Cyanobacterial Cells and Water." Master's thesis, University of Toledo, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1596824964059216

    Chicago Manual of Style (17th edition)

toledo1596824964059216
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