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The Role of SULT2 ST1 in Zebrafish Development

McElroy, Cameron Shea
http://rave.ohiolink.edu/etdc/view?acc_num=toledo1273786802

Abstract Details

2010, Master of Science in Pharmaceutical Sciences, University of Toledo, College of Pharmacy.
Cytosolic sulfotransferases are phase II drug metabolizing enzymes responsible for carrying out a sulfonation reaction which adds a sulfonyl group to a hydroxyl or amino group of its substrate. These enzymes are believed to be involved with the regulation of endogenous hormones such as neurosteroids as well as xenobiotics. Considering the increasing rates of environmental estrogen exposures, it is important to understand the role that sulfotransferases may play in the early stages of vertebrate development. SULT2 ST1 is a sulfotransferase identified in zebrafish which resembles the SULT2 A1 gene in humans. To establish a link between sulfotransferase expression and developmental toxicity, the SULT2 ST1 gene was knocked down in the zebrafish model. This was accomplished by microinjection of translation blocking morpholino oligonucleotides into <4 cell stage zebrafish embryos. The phenotypic changes observed in the SULT2 ST1 knockdown fish indicate that the absence of the enzyme may be responsible for underdeveloped fin and systemic failure in embryo development phenotypes. Western Blot was used to confirm a reduction in expressed protein in knockdown animals.
Frederick Williams, PhD (Committee Chair)
Ming-Cheh Liu, PhD (Committee Member)
Steven Peseckis, PhD (Committee Member)
60 p.
Pharmacology; Toxicology
Sulfotransferases; SULT2 ST1; Zebrafish development; morpholino knockdown

Recommended Citations

Citations

  • McElroy, C. S. (2010). The Role of SULT2 ST1 in Zebrafish Development [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1273786802

    APA Style (7th edition)

  • McElroy, Cameron. The Role of SULT2 ST1 in Zebrafish Development. 2010. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=toledo1273786802.

    MLA Style (8th edition)

  • McElroy, Cameron. "The Role of SULT2 ST1 in Zebrafish Development." Master's thesis, University of Toledo, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1273786802

    Chicago Manual of Style (17th edition)

toledo1273786802
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© 2010, all rights reserved.
This open access ETD is published by University of Toledo and OhioLINK.