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Roles of macropinocytosis and the STC1 gene in internalization of extracellular ATP, proliferation, and metastasis in human cancer cells

Stone, Nicole Alexandra
http://orcid.org/0009-0004-1409-3946
http://rave.ohiolink.edu/etdc/view?acc_num=ouashonors1685903779728055

Abstract Details

2023, Bachelor of Sciences, Ohio University, Biological Sciences.
Globally, metastasis causes approximately 90% of mortality in cancer, making it a leading cause of death. In the United States, in both men and women, lung cancer is the second most prevalent cancer, with over 283,000 new cases estimated to be diagnosed in 2023. Both the tumor microenvironment (TME) and macropinocytosis have been shown to play a role in invasion, proliferation, and recurrence of cancers. Dr. Xiaozhuo Chen’s lab at Ohio University studied the effects of the TME on cancer cells by performing RNA sequencing on A549. A549 are non-small cell human lung cancer (NSCLC) cells, which showed a consistent, significant upregulation of Stanniocalcin 1(STC1) gene expression when treated with extracellular ATP (eATP) and TGF-β. STC1 is a protein hormone involved in the regulation of the calcium phosphate balance, as well as ATP synthesis in mitochondria within the cell. Further studies showed that knock down of the STC1 gene led to reduced invasion and proliferation when compared to the untreated A549 cells. The aim of this project was to perform two main studies; one, to identify and assess macropinocytosis in a variety of cancer cell lines, and two, to investigate the effects of the STC1 gene on macropinocytosis. Using ATP concentration assays and IPA3 inhibition assays, macropinocytosis was examined in 11 cancer cell lines of varying cancer types. Macropinocytosis was confirmed with fluorescence microscopy by the colocalization of green fluorescent ATP and red fluorescent dextran. The impact of the knock-out of STC1 on macropinocytosis in A549 cells was investigated and quantified using ImageJ. The fluorescence microscopy study revealed that STC1 gene did play a role in macropinocytosis as predicted, which may be important for its effect on proliferation and invasion, the first step of metastasis.
Xiaozhuo Chen, PhD (Advisor)
Janet Duerr, PhD (Advisor)
40 p.
Biology; Cellular Biology; Molecular Biology
Macropinocytosis; Cancer; Biology; Cancer Metabolism; Lung Cancer; Stanniocalcin 1; Fluorescence Microscopy; Cancer Biology; ATP; Adenosine Triphosphate

Recommended Citations

Citations

  • Stone, N. A. (2023). Roles of macropinocytosis and the STC1 gene in internalization of extracellular ATP, proliferation, and metastasis in human cancer cells [Undergraduate thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ouashonors1685903779728055

    APA Style (7th edition)

  • Stone, Nicole. Roles of macropinocytosis and the STC1 gene in internalization of extracellular ATP, proliferation, and metastasis in human cancer cells. 2023. Ohio University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ouashonors1685903779728055.

    MLA Style (8th edition)

  • Stone, Nicole. "Roles of macropinocytosis and the STC1 gene in internalization of extracellular ATP, proliferation, and metastasis in human cancer cells." Undergraduate thesis, Ohio University, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=ouashonors1685903779728055

    Chicago Manual of Style (17th edition)

ouashonors1685903779728055
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© 2023, all rights reserved.
This open access ETD is published by Ohio University Art and Sciences Honors Theses and OhioLINK.