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Evaluating the protective efficacy of chitosan-nanoparticle based adjuvanted oral Salmonella subunit vaccine against bacterial colonization in broilers

Suresh, Raksha
http://rave.ohiolink.edu/etdc/view?acc_num=osu1720648453471609

Abstract Details

2024, Master of Science, Ohio State University, Animal Sciences.
Salmonellosis continues to be one of the major public health concerns worldwide causing a gastrointestinal disease. Poultry meat and eggs are recognised as one of the major sources of Salmonella food poisoning in humans. Our study evaluated the protective efficacy of mannose-conjugated chitosan-nanoparticle (mChitosan-NP) based oral subunit vaccine consisting of outer membrane proteins and flagella of Salmonella Enteritidis against Salmonella colonization in the intestines of broilers by incorporating two known mucosal adjuvants, c-di-GMP (stimulator of interferon gene agonist) and whole cell lysate (WCL) of Mycobacterium smegmatis. We try to identify the optimal dose of c-di-GMP and WCL adjuvants by using three different amounts (2.5µg, 10µg and 50µg/dose) in vivo to potentiate the efficacy of Salmonella subunit vaccine formulation. In vitro analysis revealed that mChitosan-NP Salmonella vaccine and mChitosan-NP adjuvant formulations were carrying high positive charge (Zeta potential +20-25mV), size 235-260nm, and polydispersity index 0.35-0.52, conducive for in vivo studies. Subsequently, the vaccine-adjuvant formulations were evaluated for efficacy in vivo in broiler chickens by challenging with Salmonella Enteritidis. Our data showed that mChitosan (OMP+FLA)/FLA-NP WCL 10µg/dose formulation reduced over 0.5 log10 reduction of challenge bacterial load comparable to a commercial live vaccine at both day post challenge 4 and 10. The systemic and mucosal antibody responses were found superior in adjuvanted mChitosan-NP Salmonella vaccine groups. Additionally, most of the vaccine groups had an increased frequency of B cells compared to mock group at day post-challenge 4, associated with upregulation of TGF-β mRNA at day post-challenge 10. Overall, mChitosan (OMP+FLA)/FLA-NP WCL 10 µg/dose and mChitosan (OMP+FLA)/FLA-NP GMP 50µg/dose performed well in inducing immune responses and efficacy.
Renukaradhya Gourapura (Advisor)
Liesa Bielke (Committee Member)
Gireesh Rajashekara (Committee Member)
63 p.
Animal Sciences
Salmonella, chitosan, subunit vaccine, nanoparticle vaccine, salmonellosis in poultry

Recommended Citations

Citations

  • Suresh, R. (2024). Evaluating the protective efficacy of chitosan-nanoparticle based adjuvanted oral Salmonella subunit vaccine against bacterial colonization in broilers [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1720648453471609

    APA Style (7th edition)

  • Suresh, Raksha. Evaluating the protective efficacy of chitosan-nanoparticle based adjuvanted oral Salmonella subunit vaccine against bacterial colonization in broilers. 2024. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1720648453471609.

    MLA Style (8th edition)

  • Suresh, Raksha. "Evaluating the protective efficacy of chitosan-nanoparticle based adjuvanted oral Salmonella subunit vaccine against bacterial colonization in broilers." Master's thesis, Ohio State University, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=osu1720648453471609

    Chicago Manual of Style (17th edition)

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This open access ETD is published by The Ohio State University and OhioLINK.