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Master's Thesis McGowan Final v2.pdf (883.74 KB)

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Utilizing an Organ-on-a-Chip to Study the Introduction of a Fecal Treatment at Hyperthermic Conditions

McGowan, Sean Patrick
http://rave.ohiolink.edu/etdc/view?acc_num=osu1713176364230623

Abstract Details

2024, Master of Science, Ohio State University, Microbiology.
The human gut microbiome is the collection of the microbiota that reside in the human intestinal tract. Imbalances in the gut microbiome are associated with multiple diseases, so studying this is important for preventing and treating these conditions. These imbalances can have multiple causes, such as changes in core temperature. Previous work has indicated that the gut microbiome could play a role in mitigating negative effects of temperature on epithelial tissues, which could have profound effects on human health. Research into the human gut is normally performed by way of animal models, or by using a synthetic model involving the use of human cells on transwells. The goal of this study was to collect preliminary data to determine if gene expression in gut epithelial cells is influenced by the presence of a fecal sample at hypoxic, hyperthermic conditions. An experiment was performed on 36 gut-on-a-chips over the course of 48 hours at three different temperature levels: 30°C, 37°C, and 42°C. Next-Generation Sequencing (NGS) was performed to determine gene expression in the human epithelial cells when comparing the introduction of a fecal sample to the chip in low-oxygen conditions at 42°C. Most genes in the host cells were upregulated when exposed to the fecal sample, with the majority being involved in immune system responses, as well as cell growth and differentiation, host metabolism, and enzymatic activity, which is consistent with what would be expected when the gut bacteria are present. The gut-on-a-chip can be used to study temperature effects on the human epithelial cells, as well as test ways to counteract any negative effects that come with this shift. Future studies can elucidate the role that the gut microbiome may play in the response of the human body to changes in core temperature.
Karen Dannemiller (Committee Chair)
Joshua Hagen (Committee Member)
Justin North (Committee Member)
73 p.
Biology
Gut Microbiome; Gut; Microbiome; Organ-on-a-chip; organ on a chip; chip; sequencing; temperature; hyperthermia; bacteria; microbes; genomes; genome; library pre; RNA; DNA

Recommended Citations

Citations

  • McGowan, S. P. (2024). Utilizing an Organ-on-a-Chip to Study the Introduction of a Fecal Treatment at Hyperthermic Conditions [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1713176364230623

    APA Style (7th edition)

  • McGowan, Sean. Utilizing an Organ-on-a-Chip to Study the Introduction of a Fecal Treatment at Hyperthermic Conditions. 2024. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1713176364230623.

    MLA Style (8th edition)

  • McGowan, Sean. "Utilizing an Organ-on-a-Chip to Study the Introduction of a Fecal Treatment at Hyperthermic Conditions." Master's thesis, Ohio State University, 2024. http://rave.ohiolink.edu/etdc/view?acc_num=osu1713176364230623

    Chicago Manual of Style (17th edition)

osu1713176364230623
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This open access ETD is published by The Ohio State University and OhioLINK.