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Milk and blood composition during subclinical mastitis

Gammariello, Corienne Sarah
http://orcid.org/0009-0005-7188-7408
http://rave.ohiolink.edu/etdc/view?acc_num=osu1689870976941036

Abstract Details

2023, Master of Science, Ohio State University, Animal Sciences.
Mastitis induces losses in milk yield and undesirable changes in milk composition. The mechanisms behind reduced milk production during mastitis remains unknown. Two studies were conducted to investigate the effects of sterile inflammation on mammary gland metabolism via changes in milk and venous blood composition. In the first study, one udder half of each primiparous Holstein cow (n = 4) in mid-lactation was infused with oyster glycogen; the other control udder half with saline. Cows were milked in tie stalls every 8 h for 5 d. Udder half milk samples were collected for analysis. Results showed milk of oyster glycogen udder halves had an increased and sustained recruitment of immune cells, increased concentrations of protein, and decreased concentrations of lactose compared to control udder halves. There were no detectable changes in fat concentrations or milk yield between udder half treatments. In the second study, primiparous Holstein cows (n = 4) were fitted with venous catheters in the left and right subcutaneous abdominal veins. Two billion cfu of formalin-fixed Staph. aureus were subsequently infused into one udder halve (FX-STAPH) of each cow; the other udder half was infused with saline (SAL). After FX-STAPH challenge, blood sampling commenced every 2.6 h till the end of the study and blood samples were analyzed using a portable blood analyzer (iStat Alinity) while plasma samples were stored for wet lab chemistry. Milk of FX-STAPH udder halves had an increased and sustained SCS, increased concentrations of protein, and decreased concentrations of lactose compared to control udder halves. Milk of FX-STAPH udder halves had increased concentrations of lactate compared to SAL udder halves. There were no changes in fat concentrations or milk yield between udder half treatments in the second study. Venous blood of FX-STAPH udder halves had marginally greater saturated and partial pressures of oxygen, greater anion gap values, and concentrations of glucose, but had a lower pH than SAL udder halves. Results indicated mammary gland metabolism changes occur during sterile leukocyte recruitment as represented by the changes in milk components and blood metabolites. This may indicate the unavailability of blood nutrients for milk synthesis in mammary glands during acute mastitis.
Benjamin Enger (Advisor)
Alejandro Relling (Committee Member)
Anja Sipka (Committee Member)
Animal Diseases; Animal Sciences; Animals
Mastitis, milk synthesis, innate immunity

Recommended Citations

Citations

  • Gammariello, C. S. (2023). Milk and blood composition during subclinical mastitis [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1689870976941036

    APA Style (7th edition)

  • Gammariello, Corienne. Milk and blood composition during subclinical mastitis. 2023. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1689870976941036.

    MLA Style (8th edition)

  • Gammariello, Corienne. "Milk and blood composition during subclinical mastitis." Master's thesis, Ohio State University, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=osu1689870976941036

    Chicago Manual of Style (17th edition)

osu1689870976941036
101
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