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Full text release has been delayed at the author's request until December 18, 2025

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Transcriptome-wide Identification of mRNA Targets of Nanos

Marhabaie, Mohammad
http://orcid.org/0000-0002-5609-2915
http://rave.ohiolink.edu/etdc/view?acc_num=osu1606852603283337

Abstract Details

2020, Doctor of Philosophy, Ohio State University, Molecular Genetics.
Translational regulation plays an important role in gene expression. In this work, I focus on Nanos (Nos), a conserved RNA-binding protein that acts as a translational repressor to control a number of biological processes. In Chapter 1, I summarize current knowledge about the mechanism of action of Nos and briefly discuss the known regulatory targets of Nos. By utilizing Upf1-Nos chimera protein, I aimed to identify mRNA regulatory targets of Nos. As described in Chapter 2, I show that Nos potentially regulates approximately 32% of the transcriptome in the early embryo. I demonstrate that the majority of Upf1-Nos depleted mRNAs are likely regulated by the cooperative action of Nos and Pumilio (Pum) in Chapter 2. I identify molecular mechanisms for recognition of this diverse population of mRNAs in Chapter 3. In Chapter 4, I describe evidence suggesting that Bruno may be a novel partner that mediates a minor fraction of Nos activity in vivo. This work dramatically expands the list of potential Nos regulatory targets, from the previous number of 11 to 2554.
Robin Wharton, Ph.D. (Advisor)
Daniel Schoenberg, Ph.D. (Committee Member)
Helen Chamberlin, Ph.D. (Committee Member)
Guramrit Singh, Ph.D. (Committee Member)
152 p.
Biology; Developmental Biology; Genetics; Molecular Biology
Nanos; Pumilio; mRNA target; Drosophila; melanogaster; Nos; Pum; Bruno;

Recommended Citations

Citations

  • Marhabaie, M. (2020). Transcriptome-wide Identification of mRNA Targets of Nanos [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1606852603283337

    APA Style (7th edition)

  • Marhabaie, Mohammad. Transcriptome-wide Identification of mRNA Targets of Nanos. 2020. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1606852603283337.

    MLA Style (8th edition)

  • Marhabaie, Mohammad. "Transcriptome-wide Identification of mRNA Targets of Nanos." Doctoral dissertation, Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1606852603283337

    Chicago Manual of Style (17th edition)

osu1606852603283337
© 2020, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.
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