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Full text release has been delayed at the author's request until August 02, 2025

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Recapitulating Brain Tumor Microenvironment with In Vitro Engineered Models

Cui, Yixiao
http://rave.ohiolink.edu/etdc/view?acc_num=osu1595545538654859

Abstract Details

2020, Doctor of Philosophy, Ohio State University, Biomedical Engineering.
Tumor microenvironment (TME) is the local environment surrounding the tumor region and can interact with tumor cells to regulate a variety of tumor behaviors. In glioblastoma (GB), emerging evidences have shown that different components in the TME can interact with tumor cells and affect tumor cell proliferation, migration, and resistance to therapy. Therapies have been developed to target GB-TME interactions but received dismal improvements on patient outcome. Currently, animal models remain to be the gold standard in preclinical drug discovery and testing. However, animal models are expensive, time consuming, and feature an overcomplex TME, making it difficult to identify specific interplays between TME and tumor cells. In vitro models incorporating well-defined TME components have been introduced as a cost-effective tool to examine tumor-TME interactions. In this dissertation, we describe four engineered models designed to recapitulate critical players in the GB TME, including the extracellular matrix, stromal cells, compressive solid stress, and interstitial fluid pressure. Using these models, we successfully captured GB cell responses to the changes in the TME and associated such responses to molecular signaling pathways. We further present a novel cell labeling method which enables multiplex antigen labeling and offers a high throughput platform for cancer diagnosis.
Jessica Winter (Advisor)
Otero Jose (Committee Member)
Ghadiali Samir (Committee Member)
Leight Jennifer (Committee Member)
293 p.
Biomedical Engineering
Glioblastoma; in vitro model design; hydrogel

Recommended Citations

Citations

  • Cui, Y. (2020). Recapitulating Brain Tumor Microenvironment with In Vitro Engineered Models [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1595545538654859

    APA Style (7th edition)

  • Cui, Yixiao. Recapitulating Brain Tumor Microenvironment with In Vitro Engineered Models. 2020. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1595545538654859.

    MLA Style (8th edition)

  • Cui, Yixiao. "Recapitulating Brain Tumor Microenvironment with In Vitro Engineered Models." Doctoral dissertation, Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1595545538654859

    Chicago Manual of Style (17th edition)

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This open access ETD is published by The Ohio State University and OhioLINK.