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JGregorich MS 7.17.pdf (1007.56 KB)

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Effects of Induced Moisture Loss on Broiler Chicks Immune Response Post Salmonella enteritidis Lipopolysaccharide Challenge

Gregorich, Jenna L
http://rave.ohiolink.edu/etdc/view?acc_num=osu149985847613047

Abstract Details

2017, Master of Science, Ohio State University, Animal Sciences.
ABSTRACT It is well understood that above optimum temperatures during incubation can lead to increased moisture loss and dehydrated chicks. For the experiments reported herein, moisture loss was measured without the confounding effects of temperature. Moisture loss was induced in chick embryos from days 11 through hatch by drilling 2 small holes in the air sac of eggs. On the day of hatch, chicks were further divided into three treatment groups, two controls and one treatment consisting of an injection with Salmonella enteritidis lipopolysaccharide (LPS). The Salmonella LPS is derived from the outer membrane of this gram-negative bacteria which is commonly found throughout poultry houses (Wang et al., 2003). The LPS injection was used to mimic a potential bacterial challenge to trigger a response from the chick’s immune system. The two control treatments consisted of one group receiving a PBS injection alone (no LPS) and a non-injected control group of eggs. At 24 and 48 hours post injection, tissue samples were collected for RT-PCR analysis of the interleukin 1 (IL1) and interleukin 10 (IL10) response in chicks with normal and increased moisture loss during incubation. The objectives of this study were to 1.) Measure moisture loss differences in egg and chick weights between the treatment groups and 2.) Study effects of induced moisture loss on the immune response when a potential pathogen is introduced.
Mike Lilburn (Advisor)
Ramesh Selvaraj (Committee Member)
Francis Fluharty (Committee Member)
48 p.
Animal Sciences
Broiler Incubation, LPS Salmonella Enteritidis challenge, Poultry Immune Response, Egg weight,

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Citations

  • Gregorich, J. L. (2017). Effects of Induced Moisture Loss on Broiler Chicks Immune Response Post Salmonella enteritidis Lipopolysaccharide Challenge [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu149985847613047

    APA Style (7th edition)

  • Gregorich, Jenna. Effects of Induced Moisture Loss on Broiler Chicks Immune Response Post Salmonella enteritidis Lipopolysaccharide Challenge. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu149985847613047.

    MLA Style (8th edition)

  • Gregorich, Jenna. "Effects of Induced Moisture Loss on Broiler Chicks Immune Response Post Salmonella enteritidis Lipopolysaccharide Challenge." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu149985847613047

    Chicago Manual of Style (17th edition)

osu149985847613047
374
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This open access ETD is published by The Ohio State University and OhioLINK.